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BIONi018-A
H050815 48-4, SAMEA4451665
iPSC line
A CLIP contains information about a cell line including any
specific third party obligations relating to, for example,
licensing obligations or the donor consent which affect the
use of the cell line.
The EBiSC Access and Use Agreement must be completed along with an individual
Cell Line Information Pack for each line. Complete the EAUA and send to Contact@EBiSC.org
for countersignature. The EAUA must be fully signed before proceeding with your order.
A batch specific Certificate of Analysis will be available to
download once you receive your EBiSC iPSC line.
General#
Cell Line |
|
| hPSCreg name | BIONi018-A |
| Alternative name(s) |
H050815 48-4, SAMEA4451665
|
| Cell line type | Human induced pluripotent stem cell (hiPSC) |
| Similar lines | No similar lines found. |
Provider |
|
| Depositor | Bioneer (BION) |
| Owner | Bioneer (BSC) |
| Distributors |
EBiSC
|
| Derivation country | Denmark |
External Databases |
|
| hPSCreg | BIONi018-A |
| BioSamples | SAMEA4451665 |
| Cellosaurus | CVCL_LE16 |
| Wikidata | Q54796799 |
General Information |
|
| This EBiSC line can be used for: |
Yes
Research use: allowed
Clinical use: no
Commercial use: no
|
Donor Information#
General Donor Information |
|
| Sex | male |
| Age of donor (at collection) | 20-24 |
Phenotype and Disease related information (Donor) |
|
| Diseases | No disease was diagnosed.
|
Karyotyping (Donor) |
|
| Has the donor karyotype been analysed? |
No
|
Other Genotyping (Donor) |
|
| Is there genome-wide genotyping or functional data available? |
No
|
External Databases (Donor) |
|
| BioSamples | SAMEA4451664 |
hIPSC Derivation#
General |
|
| Source cell type |
An adult mesenchymal stem cell derived from adipose tisssue.
Synonyms
|
| Source cell origin |
Portion of connective tissue composed of adipocytes enmeshed in areolar tissue.
|
| Source cell type (free text) | Pre-Adipocyte |
| Age of donor (at collection) | 20-24 |
| Passage number reprogrammed | 3 |
Reprogramming method |
|
| Vector type | Non-integrating |
| Vector | Episomal |
| Genes | |
| Is reprogramming vector detectable? |
No |
| Methods used |
PCR
|
| Notes on reprogramming vector detection | DNA isolated at passage 5 or 6 after colony picking. Quantitative PCR with the exogenous pluripotency genes LIN28, SOX2, and OCT4 in iPSC lines. Normal human dermal fibroblasts (NHDFs) transfected two days before with hOSKUL served as a positive control and a sample without template served as negative control. |
| Vector map | |
Vector free reprogramming |
|
Other |
|
| Derived under xeno-free conditions |
Yes |
| Derived under GMP? |
No |
| Available as clinical grade? |
No |
Culture Conditions#
Latest released batch |
|
| Culture medium | Essential E8 |
| Passage method | EDTA |
| Surface coating | Matrigel / Geltrex |
| O2 concentration | 5 |
| CO2 concentration | 5 |
| Temperature | 37 |
The following are the depositor culture conditions, they do not refer to any specific batch.
| Surface coating | Matrigel/Geltrex |
| Feeder cells |
No |
| Passage method |
Enzyme-free cell dissociation
EDTA
|
| O2 Concentration | 5 % |
| CO2 Concentration | 5 % |
| Medium |
Essential 8™
|
| Has Rock inhibitor (Y27632) been used at passage previously with this cell line? | No |
| Has Rock inhibitor (Y27632) been used at cryo previously with this cell line? | No |
| Has Rock inhibitor (Y27632) been used at thaw previously with this cell line? | No |
Characterisation#
Analysis of Undifferentiated Cells
| Marker | Expressed | Immunostaining | RT-PCR | Flow Cytometry | Enzymatic Assay | Expression Profiles |
| POU5F1 (OCT-4) |
Yes |
|||||
| SOX2 |
Yes |
|
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| NANOG |
Yes |
|
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| SSEA-1 |
No |
|
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| SSEA-4 |
Yes |
|
||||
| SSEA-4 |
Yes |
|
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| TRA 1-81 |
Yes |
|
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| TDGF1 |
Yes |
|
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| GDF3 |
Yes |
|
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| GABRB3 |
Yes |
|
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| DMNT3B |
Yes |
|
Morphology pictures
Differentiation Potency
Microbiology / Virus Screening |
|
| HIV 1 | Negative |
| HIV 2 | Negative |
| Hepatitis B | Negative |
| Hepatitis C | Negative |
| Mycoplasma | Negative |
Sterility |
|
| Inoculation for microbiological growth | No Contaminants Detected |
| Mycoplasma | Not Detected |
| Viability | Viable post-cryopreservation |
Genotyping#
Karyotyping (Cell Line) |
|
| Has the cell line karyotype been analysed? |
Yes
|
Other Genotyping (Cell Line) |
|