The EBiSC team is working hard to implement improvements in how EBiSC operates.
Due to some short-term disruption, please get in touch via
Contact@EBiSC.org if
the cells you would like to access are currently listed as unavailable or you
are ordering from outside of Europe.
BCRTi010-A
iPSC line
We are currently making some changes to how EBiSC operates and because
of this there is a short period of time where orders cannot be placed.
If you are interested in accessing these cells, please contact EBiSC directly. For more information about the current transition process see here.
If you are interested in accessing these cells, please contact EBiSC directly. For more information about the current transition process see here.
The cell line is not released.
General#
Cell Line |
|
hPSCreg name | BCRTi010-A |
Cell line type | Human induced pluripotent stem cell (hiPSC) |
Similar lines | No similar lines found. |
Provider |
|
Depositor | Berlin-Brandenburg Center for Regenerative Therapies (BCRT) |
Owner | Berlin-Brandenburg Center for Regenerative Therapies (BCRT) |
Distributors |
Berlin-Brandenburg Center for Regenerative Therapies (BCRT)
EBiSC
|
Derivation country | Germany |
External Databases |
|
hPSCreg | BCRTi010-A |
BioSamples | SAMEA112641225 |
Cellosaurus | CVCL_C7RK |
Wikidata | Q117704212 |
General Information |
|
Publications | View all related publications on hPSCreg (1) |
This EBiSC line can be used for: |
Yes
Research use: allowed
Clinical use: no
Commercial use: no
|
Donor Information#
General Donor Information |
|
Sex | male |
Phenotype and Disease related information (Donor) |
|
Diseases | No disease was diagnosed.
|
Karyotyping (Donor) |
|
Has the donor karyotype been analysed? |
No
|
Other Genotyping (Donor) |
|
Is there genome-wide genotyping or functional data available? |
No
|
Donor Relations |
|
All cell lines of this donor's relatives |
Has daughter:
|
External Databases (Donor) |
|
BioSamples | SAMEA112641226 |
hIPSC Derivation#
General |
|
Source cell type |
A peripheral blood cell with a single nucleus. This category includes lymphocytes and monocytes.
Synonyms
|
Passage number reprogrammed | 10 |
Reprogramming method |
|
Vector type | Non-integrating |
Vector | Episomal |
Genes | |
Methods used |
RT-PCR
|
Files and images showing reprogramming vector expressed or silenced | |
Vector free reprogramming |
|
Type of used vector free reprogramming factor(s) |
None
|
Other |
|
Derived under xeno-free conditions |
Yes |
Derived under GMP? |
No |
Available as clinical grade? |
No |
Culture Conditions#
The following are the depositor culture conditions, they do not refer to any specific batch.
Surface coating | Matrigel/Geltrex |
Feeder cells |
No |
Passage method |
Enzymatically
EDTA-free cell dissociation EDTA
|
O2 Concentration | 21 % |
CO2 Concentration | 5 % |
Medium |
Essential 8™
|
Has Rock inhibitor (Y27632) been used at passage previously with this cell line? | Yes |
Has Rock inhibitor (Y27632) been used at cryo previously with this cell line? | Yes |
Has Rock inhibitor (Y27632) been used at thaw previously with this cell line? | Yes |
Characterisation#
Analysis of Undifferentiated Cells
Marker | Expressed | Immunostaining | RT-PCR | Flow Cytometry | Enzymatic Assay | Expression Profiles |
NANOG |
Yes |
|
||||
SSEA-4 |
Yes |
|
||||
POU5F1 (OCT-4) |
Yes |
|
||||
TRA 1-60 |
Yes |
|
||||
SOX2 |
Yes |
|
Differentiation Potency
In vitro spontaneous differentiation
Marker | Expressed |
Goosecoid |
Yes |
SOX17 |
Yes |
In vitro spontaneous differentiation
Marker | Expressed |
MIXL1 |
Yes |
Brachyuri (T) |
Yes |
In vitro spontaneous differentiation
Marker | Expressed |
OTX2 |
Yes |
NEUROD1 |
Yes |
Microbiology / Virus Screening |
|
HIV 1 | Negative |
HIV 2 | Negative |
Hepatitis B | Negative |
Hepatitis C | Negative |
Mycoplasma | Negative |
Genotyping#
Karyotyping (Cell Line) |
|
Has the cell line karyotype been analysed? |
Yes
SNP anaylsis
|
Other Genotyping (Cell Line) |