BCRTi010-A
iPSC line
The cell line is not available.
General#
Cell Line |
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| hPSCreg Name | BCRTi010-A |
| Cell line type | Human induced pluripotent stem cell (hiPSC) |
Provider |
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| Depositor | Berlin-Brandenburg Center for Regenerative Therapies (BCRT) |
| Owner | Berlin-Brandenburg Center for Regenerative Therapies (BCRT) |
| Distributors |
Berlin-Brandenburg Center for Regenerative Therapies (BCRT)
EBiSC
European Collection of Authenticated Cell Cultures (ECACC)
|
| Derivation country | Germany |
External Databases |
|
| hPSCreg | BCRTi010-A |
| BioSamples | SAMEA112641225 |
| CLO | CLO_0104206 |
| Cellosaurus | CVCL_C7RK |
General Information |
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| * Is the cell line readily obtainable for third parties? |
Yes Research use: allowed
Clinical use: allowed
Commercial use: allowed
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Donor Information#
General Donor Information |
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| Sex | male |
Phenotype and Disease related information (Donor) |
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| Diseases | No disease was diagnosed.
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Karyotyping (Donor) |
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| Has the donor karyotype been analysed? |
No
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Other Genotyping (Donor) |
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| Is there genome-wide genotyping or functional data available? |
No
|
Donor Relations |
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| All cell lines of this donor's relatives |
Has daughter:
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External Databases (Donor) |
|
| BioSamples | SAMEA112641226 |
hIPSC Derivation#
General |
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| Source cell type |
Peripheral Blood Mononuclear CellA peripheral blood cell with a single nucleus. This category includes lymphocytes and monocytes.
Synonyms
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| Passage number reprogrammed | 10 |
Reprogramming method |
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| Vector type | Non-integrating |
| Vector | Episomal |
| Genes | |
| Methods used |
RT-PCR
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| Files and images showing reprogramming vector expressed or silenced | |
Vector free reprogramming |
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| Type of used vector free reprogramming factor(s) |
None
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Other |
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| Derived under xeno-free conditions |
Yes |
| Derived under GMP? |
No |
| Available as clinical grade? |
No |
Culture Conditions#
The following are the depositor culture conditions, they do not refer to any specific batch.
| Surface coating | Matrigel/Geltrex |
| Feeder cells |
No |
| Passage method |
Enzymatically
EDTA-free cell dissociation EDTA
|
| O2 Concentration | 21 % |
| CO2 Concentration | 5 % |
| Medium |
Essential 8™
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| Has Rock inhibitor (Y27632) been used at passage previously with this cell line? | Yes |
| Has Rock inhibitor (Y27632) been used at cryo previously with this cell line? | Yes |
| Has Rock inhibitor (Y27632) been used at thaw previously with this cell line? | Yes |
Characterisation#
Analysis of Undifferentiated Cells
| Marker | Expressed | Immunostaining | RT-PCR | FACS | Enzymatic Assay | Expression Profiles |
| NANOG |
Yes |
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| SSEA-4 |
Yes |
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| POU5F1 (OCT-4) |
Yes |
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| TRA 1-60 |
Yes |
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| SOX2 |
Yes |
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Differentiation Potency
In vitro spontaneous differentiation
| Marker | Expressed |
| Goosecoid |
Yes |
| SOX17 |
Yes |
In vitro spontaneous differentiation
| Marker | Expressed |
| MIXL1 |
Yes |
| Brachyuri (T) |
Yes |
In vitro spontaneous differentiation
| Marker | Expressed |
| OTX2 |
Yes |
| NEUROD1 |
Yes |
Microbiology / Virus Screening |
|
| HIV 1 | Negative |
| HIV 2 | Negative |
| Hepatitis B | Negative |
| Hepatitis C | Negative |
| Mycoplasma | Negative |
Genotyping#
Karyotyping (Cell Line) |
|
| Has the cell line karyotype been analysed? |
Yes
SNP anaylsis
|
Other Genotyping (Cell Line) |
|