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WTSIi103-A

HPSI1213i-nusw_2

iPSC line

No longer available
We are currently making some changes to how EBiSC operates and because of this there is a short period of time where orders cannot be placed.

If you are interested in accessing these cells, please contact EBiSC directly. For more information about the current transition process see here.
The cell line was withdrawn.

General#

Cell Line

hPSCreg name WTSIi103-A
Alternative name(s)
HPSI1213i-nusw_2
Cell line type Human induced pluripotent stem cell (hiPSC)
Similar lines No similar lines found.

Provider

Depositor Wellcome Sanger Institute (WTSI)
Distributors
EBiSC
Derivation country United Kingdom

External Databases

hPSCreg WTSIi103-A
BioSamples SAMEA2464779
HipSci HPSI1213i-nusw_2
Cellosaurus CVCL_AI20
Wikidata Q54891724

General Information

Publications View all related publications on hPSCreg (2)
This EBiSC line can be used for:
Yes
Research use: allowed
Clinical use: no
Commercial use: no

Donor Information#

General Donor Information

Sex male
Age of donor (at collection) 65-69
Ethnicity White - White British

Phenotype and Disease related information (Donor)

Diseases No disease was diagnosed.

External Databases (Donor)

BioSamples SAMEA2398815
HipSci HPSI-nusw

hIPSC Derivation#

General

Source cell type
A connective tissue cell which secretes an extracellular matrix rich in collagen and other macromolecules. Flattened and irregular in outline with branching processes; appear fusiform or spindle-shaped.; These cells may be vimentin-positive, fibronectin-positive, fsp1-positive, MMP-1-positive, collagen I-positive, collagen III-positive, and alpha-SMA-negative.
Source cell origin
Any portion of the organ that covers that body and consists of a layer of epidermis and a layer of dermis.
Synonyms
  • portion of skin
  • region of skin
  • skin
  • skin region
  • skin zone
show more synonyms
Age of donor (at collection) 65-69
Collected in 2014
Source cell line vendor Cambridge BioResource

Reprogramming method

Vector type Non-integrating
Vector Sendai virus
Genes
Notes on reprogramming vector detection CytoTune 1

Vector free reprogramming

Other

Selection criteria for clones Morphology
Derived under xeno-free conditions
No
Derived under GMP?
No
Available as clinical grade?
No

Culture Conditions#

The following are the depositor culture conditions, they do not refer to any specific batch.
Surface coating Gelatin
Feeder cells Mouse embryo fibroblast (MEF) feeder cells
Passage method Enzymatically
Collagenase and Dispase
CO2 Concentration 5 %
Medium Other medium:
Base medium:
Main protein source: Knock-out serum replacement

Characterisation#

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR Flow Cytometry Enzymatic Assay Expression Profiles
POU5F1 (OCT-4)
Yes
SOX2
Yes
NANOG
Yes
Pluripotency Score Novelty Score Link to microarray data
35.818 1.861 http://www.ebi.ac.uk/arrayexpress/experiments/E-MTAB-4057/

Genotyping#

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
No

Other Genotyping (Cell Line)

Is there genome-wide genotyping or functional data available?
Yes
cnv
http://www.hipsci.org/lines/#/lines/HPSI1213i-nusw_2
Number of regions different from primary tissue: 1; Length of differences from primary tissue: 4
RNA-seq
http://www.ebi.ac.uk/ena/data/view/ERZ267026
Abundances of transcripts
Exome sequencing
http://www.ebi.ac.uk/ena/data/view/ERR780155
Raw sequencing reads
RNA-seq
http://www.ebi.ac.uk/ena/data/view/ERR946997
Raw sequencing reads
Whole genome sequencing
http://www.ebi.ac.uk/ena/data/view/ERR1860037
Raw sequencing reads
Whole genome sequencing
http://www.ebi.ac.uk/ena/data/view/ERR1859941
Raw sequencing reads
Whole genome sequencing
http://www.ebi.ac.uk/ena/data/view/ERZ448073
GATK haplotype calls
Genotyping array
http://www.ebi.ac.uk/ena/data/view/ERZ127396
Imputed and phased genotypes
RNA-seq
http://www.ebi.ac.uk/ena/data/view/ERR946972
Raw sequencing reads
Whole genome sequencing
http://www.ebi.ac.uk/ena/data/view/ERR1860805
Raw sequencing reads
Whole genome sequencing
http://www.ebi.ac.uk/ena/data/view/ERR1861093
Raw sequencing reads
Whole genome sequencing
http://www.ebi.ac.uk/ena/data/view/ERR1860901
Raw sequencing reads
Whole genome sequencing
http://www.ebi.ac.uk/ena/data/view/ERR1860709
Raw sequencing reads
Genotyping array
http://www.ebi.ac.uk/ena/data/view/ERZ127631
Genotyping array calls
Exome sequencing
http://www.ebi.ac.uk/ena/data/view/ERZ122995
Imputed and phased genotypes
Whole genome sequencing
http://www.ebi.ac.uk/ena/data/view/ERR1860421
Raw sequencing reads
RNA-seq
http://www.ebi.ac.uk/ena/data/view/ERZ123062
Splice-aware STAR alignment
Exome sequencing
http://www.ebi.ac.uk/ena/data/view/ERR847009
Raw sequencing reads
Methylation profiling
http://www.ebi.ac.uk/arrayexpress/experiments/E-MTAB-4059/
Text file with probe intensities
Whole genome sequencing
http://www.ebi.ac.uk/ena/data/view/ERR1861285
Raw sequencing reads
Whole genome sequencing
http://www.ebi.ac.uk/ena/data/view/ERR1861189
Raw sequencing reads
Exome sequencing
http://www.ebi.ac.uk/ena/data/view/ERZ122929
mpileup variant calls
Whole genome sequencing
http://www.ebi.ac.uk/ena/data/view/ERR1860997
Raw sequencing reads
Whole genome sequencing
http://www.ebi.ac.uk/ena/data/view/ERR1860613
Raw sequencing reads
Whole genome sequencing
http://www.ebi.ac.uk/ena/data/view/ERR1860517
Raw sequencing reads
WGS-derived disease associations
3-hydroxyisobutyryl-CoA hydrolase deficiency (HIBCH)
Bernard-Soulier syndrome (GP1BA)
Brugada syndrome 1 (CACNB2)
Charcot-Marie-Tooth disease (FIG4)
Cohen syndrome (VPS13B)
complex neurodevelopmental disorder (CNTNAP2, SETBP1)
demyelinating hereditary motor and sensory neuropathy (MTMR2)
dilated cardiomyopathy (NPPA)
familial ovarian cancer (GEN1)
Fanconi anemia complementation group A (FANCA)
hereditary breast carcinoma (GEN1)
hereditary nonpolyposis colon cancer (XRCC4)
hypertrophic cardiomyopathy (CACNB2)
immunodeficiency, common variable, 3 (CD19)
Leigh syndrome (HIBCH)
mitochondrial disease (NDUFS6)
monogenic diabetes (PAX4)
nephronophthisis 4 (NPHP4)
nonsyndromic genetic hearing loss (CDH23, MYO7A)
PHARC syndrome (ABHD12)
platelet-type von Willebrand disease (GP1BA)
PTEN hamartoma tumor syndrome (PTEN)
renal tubular acidosis, distal, 2, with progressive sensorineural hearing loss (ATP6V1B1)
Rothmund-Thomson syndrome (RECQL4)
Schinzel-Giedion syndrome (SETBP1)
short QT syndrome (CACNB2)
syndromic intellectual disability (KMT2C)
turnpenny-fry syndrome (PCGF2)
Usher syndrome type 1 (CDH23, MYO7A)
Other WGS-derived genes
AGAP1, AGL, ALDH3A2, AMPD1, ANAPC1, ATP8B3, AURKC, C6, CASP12, CEP170, CHIT1, CHST15, CNOT1, COLQ, CYP21A2, D2HGDH, DSC3, ERCC6L2, FBXO7, FLG2, FUT2, GALNT3, GDPD4, HSD17B13, ITGB2, KCNJ16, LAMA5, LPL, MAPT, MESP1, MROH8, NDUFB9, NOTCH2, NPRL3, OAS1, OR1B1, OR51F1, OR52B4, P2RX5, PDE4DIP, PIGN, POLDIP2, RNF212, SCAPER, SELPLG, SIGLEC12, SLC37A4, SPTBN5, SYNE2, TAP2, TBP, TIGD6, TLR5, TMEM107, TMEM216, TNRC18, TOR1AIP1, TREH, TRPM1, VDR, VRK1, WDR37, ZAN, ZNF141, ZNF83, ZNF844