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WTSIi009-A

HPSI0913i-diku_1

iPSC line

Immediately available for distribution*
*Once all legal and processing details completed
We are currently making some changes to how EBiSC operates and because of this there is a short period of time where orders cannot be placed.

If you are interested in accessing these cells, please contact EBiSC directly. For more information about the current transition process see here.
A CLIP contains information about a cell line including any specific third party obligations relating to, for example, licensing obligations or the donor consent which affect the use of the cell line.

The EBiSC Access and Use Agreement must be completed along with an individual Cell Line Information Pack for each line. Complete the EAUA and send to Contact@EBiSC.org for countersignature. The EAUA must be fully signed before proceeding with your order.
A batch specific Certificate of Analysis will be available to download once you receive your EBiSC iPSC line.

General#

Cell Line

hPSCreg name WTSIi009-A
Alternative name(s)
HPSI0913i-diku_1
Cell line type Human induced pluripotent stem cell (hiPSC)
Similar lines No similar lines found.

Provider

Depositor Wellcome Sanger Institute (WTSI)
Distributors
EBiSC
Derivation country United Kingdom

External Databases

hPSCreg WTSIi009-A
BioSamples SAMEA2593858
HipSci HPSI0913i-diku_1
Cellosaurus CVCL_AH28
Wikidata Q54891443

General Information

Publications View all related publications on hPSCreg (1)
This EBiSC line can be used for:
Yes
Research use: allowed
Clinical use: no
Commercial use: no
Subclones

Donor Information#

General Donor Information

Sex female
Age of donor (at collection) 60-64
Ethnicity White - White British

Phenotype and Disease related information (Donor)

Diseases No disease was diagnosed.

External Databases (Donor)

BioSamples SAMEA2398076
HipSci HPSI-diku

hIPSC Derivation#

General

Source cell type
A connective tissue cell which secretes an extracellular matrix rich in collagen and other macromolecules. Flattened and irregular in outline with branching processes; appear fusiform or spindle-shaped.; These cells may be vimentin-positive, fibronectin-positive, fsp1-positive, MMP-1-positive, collagen I-positive, collagen III-positive, and alpha-SMA-negative.
Source cell origin
Any portion of the organ that covers that body and consists of a layer of epidermis and a layer of dermis.
Synonyms
  • portion of skin
  • region of skin
  • skin
  • skin region
  • skin zone
show more synonyms
Age of donor (at collection) 60-64
Collected in 2014
Source cell line vendor Cambridge BioResource

Reprogramming method

Vector type Non-integrating
Vector Sendai virus
Genes
Notes on reprogramming vector detection CytoTune 1

Vector free reprogramming

Other

Selection criteria for clones Morphology
Derived under xeno-free conditions
No
Derived under GMP?
No
Available as clinical grade?
No

Culture Conditions#

Latest released batch

Culture medium Essential E8
Passage method EDTA
Surface coating Vitronectin
O2 concentration 21
CO2 concentration 5
Temperature 37
The following are the depositor culture conditions, they do not refer to any specific batch.
Surface coating Vitronectin
Feeder cells
No
Passage method Enzyme-free cell dissociation
EDTA
CO2 Concentration 5 %
Medium TeSR™ E8™

Characterisation#

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR Flow Cytometry Enzymatic Assay Expression Profiles
POU5F1 (OCT-4)
Yes
SOX2
Yes
NANOG
Yes
SSEA-4
Yes
SSEA-1
No
TRA 1-60
Yes
Pluripotency Score Novelty Score Link to microarray data
28.091 1.259 http://www.ebi.ac.uk/arrayexpress/experiments/E-MTAB-4057/

Microbiology / Virus Screening

HIV 1 Negative
HIV 2 Negative
Hepatitis B Negative
Hepatitis C Negative
Mycoplasma Negative

Sterility

Inoculation for microbiological growth No Contaminants Detected
Mycoplasma Not Detected
Viability Viable post-cryopreservation

Genotyping#

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
No

Other Genotyping (Cell Line)

Is there genome-wide genotyping or functional data available?
Yes
cnv
http://www.hipsci.org/lines/#/lines/HPSI0913i-diku_1
Number of regions different from primary tissue: 0; Length of differences from primary tissue: 0
RNA-seq
http://www.ebi.ac.uk/ena/data/view/ERR914340
Raw sequencing reads
Whole genome sequencing
http://www.ebi.ac.uk/ena/data/view/ERR1861318
Raw sequencing reads
RNA-seq
http://www.ebi.ac.uk/ena/data/view/ERZ267009
Abundances of transcripts
Exome sequencing
http://www.ebi.ac.uk/ena/data/view/ERZ123023
Imputed and phased genotypes
Genotyping array
http://www.ebi.ac.uk/ena/data/view/ERZ127607
Genotyping array calls
RNA-seq
http://www.ebi.ac.uk/ena/data/view/ERR914304
Raw sequencing reads
Exome sequencing
http://www.ebi.ac.uk/ena/data/view/ERR947069
Raw sequencing reads
Genotyping array
http://www.ebi.ac.uk/ena/data/view/ERZ127372
Imputed and phased genotypes
RNA-seq
http://www.ebi.ac.uk/ena/data/view/ERZ123090
Splice-aware STAR alignment
Whole genome sequencing
http://www.ebi.ac.uk/ena/data/view/ERZ448008
GATK haplotype calls
Exome sequencing
http://www.ebi.ac.uk/ena/data/view/ERZ122957
mpileup variant calls
Exome sequencing
http://www.ebi.ac.uk/ena/data/view/ERR947061
Raw sequencing reads
Methylation profiling
http://www.ebi.ac.uk/arrayexpress/experiments/E-MTAB-4059/
Text file with probe intensities
Whole genome sequencing
https://ega-archive.org/studies/EGAS00001002755
This cell line has undergone WGS using the Illumina HiSeq X platform at 30x coverage. Fastq files are stored at the European Genome Archive, users can apply for access to this data by submitting an application form to the EBiSC Data Access Committee https://ega-archive.org/dacs/EGAC00001000768
WGS-derived disease associations
3-hydroxyisobutyryl-CoA hydrolase deficiency (HIBCH)
Charcot-Marie-Tooth disease (FIG4, HINT1)
Cohen syndrome (VPS13B)
complex neurodevelopmental disorder (CNTNAP2, LAMC3, SETBP1)
demyelinating hereditary motor and sensory neuropathy (MTMR2)
familial ovarian cancer (GEN1)
familial thoracic aortic aneurysm and aortic dissection (MFAP5)
hereditary breast carcinoma (GEN1)
hereditary nonpolyposis colon cancer (XRCC4)
Hermansky-Pudlak syndrome 4 (HPS4)
Leigh syndrome (HIBCH)
mitochondrial disease (NDUFS6)
monogenic diabetes (PAX4)
nephronophthisis 4 (NPHP4)
nonsyndromic genetic hearing loss (CDH23, MYO7A)
primary ciliary dyskinesia 7 (DNAH11)
renal tubular acidosis, distal, 2, with progressive sensorineural hearing loss (ATP6V1B1)
Rothmund-Thomson syndrome (RECQL4)
Schinzel-Giedion syndrome (SETBP1)
Smith-Magenis syndrome (RAI1)
syndromic intellectual disability (KMT2C)
Usher syndrome type 1 (CDH23, MYO7A)
X-linked complex neurodevelopmental disorder (ZNF711)
Other WGS-derived genes
A2M, AGL, AGMO, ALDH3A2, AMPD1, ANAPC1, ARMS2, AURKC, BMP4, CASP12, CATSPER2, CHST15, CLDN16, COLQ, CST3, CYP21A2, CYP4B1, D2HGDH, DEFB126, DNAAF1, DNAJA4, DSC3, ERCC6L2, FCGR2A, FLG2, FUT2, FZD6, GALNT3, GBE1, GDPD4, GPRC6A, HLA-DRB5, HSD17B13, IDO2, IRF5, ITGB2, KCNJ16, KISS1, MESP1, MICA, MROH8, MSR1, MTTP, NDUFB9, NFU1, OAS1, OPRM1, OTOR, P2RX5, PDE4DIP, PIGN, POLDIP2, PRKRA, PTCHD3, RXFP2, SCAPER, SIGLEC12, SLC37A4, SPATA7, SRA1, STAG2, TBP, TIGD6, TMEM107, TMEM216, TNRC18, TOR1AIP1, TREH, TRPM1, VDR, WDR37, ZAN, ZNF141, ZNF233, ZNF527