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ESi007-A

CBiPS1sv-4F-40

iPSC line

Available directly from depositor
Timepoint: Confluence
Magnification: 4x
Timepoint: Confluence
Magnification: 10x
You will need to complete a Cell Line Information Pack (CLIP) before purchasing your cell line. Please contact us to receive the relevant document.

The EBiSC Access and Use Agreement must be completed along with an individual Cell Line Information Pack for each line. Complete the EAUA and send to Contact@EBiSC.org for countersignature. The EAUA must be fully signed before proceeding with your order.
A batch specific Certificate of Analysis will be available to download once you receive your EBiSC iPSC line.

General#

Cell Line

hPSCreg name ESi007-A
Alternative name(s)
CBiPS1sv-4F-40
Cell line type Human induced pluripotent stem cell (hiPSC)
Similar lines No similar lines found.
Notes Please note that the correct synonym is "CBiPS1sv-4F-40", not "CBiPSsv-4F-40" as stated in PMID:30778227. [NM]

Provider

Depositor Spanish Stem Cell Bank (ES)
Owner Inbiomed
Distributors
EBiSC
Inbiomed
Derivation country Spain

External Databases

hPSCreg ESi007-A
BioSamples SAMEA3303231
Cellosaurus CVCL_V189
Wikidata Q54808661

General Information

Publications View all related publications on hPSCreg (3)
This EBiSC line can be used for:
Yes
Research use: allowed
Clinical use: no
Commercial use: no

Donor Information#

General Donor Information

Sex female
Age of donor (at collection) neonate

Phenotype and Disease related information (Donor)

Diseases No disease was diagnosed.

External Databases (Donor)

BioSamples SAMEA3303248

hIPSC Derivation#

General

Source cell type
Any hematopoietic stem cell that is part of a umbilical cord blood.
Source cell origin
Blood that remains in the placenta and in the attached umbilical cord after childbirth[WP].; Cord blood is collected because it contains stem cells, which can be used to treat hematopoietic and genetic disorders.[WP]
Synonyms
  • cord blood
  • fetal blood
  • umbilical cord blood
Source cell type (free text) Cord blood CD133+
Age of donor (at collection) neonate

Reprogramming method

Vector type Non-integrating
Vector Sendai virus
Genes
Is reprogramming vector detectable?
No
Methods used
RT-PCR

Vector free reprogramming

Other

Selection criteria for clones morphological
Derived under xeno-free conditions
No
Derived under GMP?
No
Available as clinical grade?
No

Culture Conditions#

Latest released batch

Culture medium mTeSR
Passage method EDTA
Surface coating Matrigel / Geltrex
O2 concentration 21
CO2 concentration 5
Temperature 37
The following are the depositor culture conditions, they do not refer to any specific batch.
Surface coating Gelatin
Feeder cells Mouse embryonic fibroblasts
Cellfinder Ont Id: EFO_0004040
Passage method Mechanically
CO2 Concentration 5 %
Medium Other medium:
Base medium: Knockout DMEM
Main protein source: Knock-out serum replacement
Serum concentration: 20 %
Supplements
NEAA 1 %
Glutamax 2 mM
2-mercaptoethanol 0,05 %
bFGF 10 ng/ml
Penicillin Streptomycin 0,5 %

Characterisation#

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR Flow Cytometry Enzymatic Assay Expression Profiles
Alkaline Phosphatase
Yes
NANOG
Yes
POU5F1 (OCT-4)
Yes
SSEA-3
Yes
TRA 1-60
Yes
ZFP42 (REX-1)
Yes
SOX2
Yes
Differentiation Potency
Endoderm
Ont Id: UBERON_0000925
In vivo teratoma
In vitro spontaneous differentiation
Marker Expressed
FOXA2
Yes
AFP
Yes
SOX17
Yes
Mesoderm
Ont Id: UBERON_0000926
In vivo teratoma
In vitro spontaneous differentiation
Marker Expressed
SMA
Yes
MEOX
Yes
AAP
Yes
Ectoderm
Ont Id: UBERON_0000924
In vivo teratoma
In vitro spontaneous differentiation
Marker Expressed
Tuj1
Yes
PAX6
Yes
NeuroD3
Yes
Sox1
Yes

Microbiology / Virus Screening

HIV 1 Negative
HIV 2 Negative
Hepatitis B Negative
Hepatitis C Negative
Mycoplasma Negative

Genotyping#

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
Yes
46,XX
Karyotyping method: G-Banding

Other Genotyping (Cell Line)

Is there genome-wide genotyping or functional data available?
Yes