WTSIi105-D
HPSI0313i-airc_6
iPSC line
This (hPSCreg) cell line is not part of EBiSC.
General#
Cell Line |
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hPSCreg Name | WTSIi105-D |
Alternative name(s) |
HPSI0313i-airc_6
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Cell line type | Human induced pluripotent stem cell (hiPSC) |
Provider |
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Depositor | Wellcome Trust Sanger Institute (WTSI) |
External Databases |
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hPSCreg | WTSIi105-D |
BioSamples | SAMEA2398072 |
HipSci | HPSI0313i-airc_6 |
Cellosaurus | CVCL_AE69 |
CLO | CLO_0101093 |
Wikidata | Q54890597 |
General Information |
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* Is the cell line readily obtainable for third parties? |
Yes Research use: allowed
Clinical use: not allowed
Commercial use: not allowed
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Donor Information#
General Donor Information |
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Sex | female |
Phenotype and Disease related information (Donor) |
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Diseases | No disease was diagnosed.
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Donor Relations |
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Other cell lines of this donor |
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External Databases (Donor) |
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BioSamples | SAMEA2398654 |
HipSci | HPSI-airc |
hIPSC Derivation#
General |
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Source cell type |
fibroblastA connective tissue cell which secretes an extracellular matrix rich in collagen and other macromolecules. Flattened and irregular in outline with branching processes; appear fusiform or spindle-shaped.
|
Source cell origin |
zone of skinAny portion of the organ that covers that body and consists of a layer of epidermis and a layer of dermis.
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Source cell line vendor | Cambridge BioResource |
Reprogramming method |
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Vector type | Non-integrating |
Vector | Episomal |
Vector free reprogramming |
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Other |
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Derived under xeno-free conditions |
Unknown |
Derived under GMP? |
Unknown |
Available as clinical grade? |
Unknown |
Culture Conditions#
The following are the depositor culture conditions, they do not refer to any specific batch.
Feeder cells |
Mouse embryo fibroblast (MEF) feeder cells |
Passage method |
Enzymatically
Collagenase and Dispase
|
CO2 Concentration | 5 % |
Medium |
Other medium:
Base medium:
Main protein source: Knock-out serum replacement Serum concentration: % |
Characterisation#
Analysis of Undifferentiated Cells
Pluripotency Score | Novelty Score | |
45.478 | 1.319 |
Report
HPSI-airc.pluritest.pluripotency_score.20161010.png
pluripotency image
HPSI-airc.pluritest.novelty_score.20161010.png
novelty image
Genotyping#
Karyotyping (Cell Line) |
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Has the cell line karyotype been analysed? |
No
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Other Genotyping (Cell Line) |
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Is there genome-wide genotyping or functional data available? |
Yes
cnv
http://www.hipsci.org/lines/#/lines/HPSI0313i-airc_6 Number of regions different from primary tissue: 0; Length of differences from primary tissue: 0 |