UCLi006-A
LCMD-L302P-UCL01C2
iPSC line
The cell line is not available.
General#
Cell Line |
|
hPSCreg Name | UCLi006-A |
Alternative name(s) |
LCMD-L302P-UCL01C2
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Cell line type | Human induced pluripotent stem cell (hiPSC) |
Similar lines | |
Provider |
|
Depositor | University College London (UCL) |
Distributors |
EBiSC
European Collection of Authenticated Cell Cultures (ECACC)
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Derivation country | United Kingdom |
External Databases |
|
hPSCreg | UCLi006-A |
BioSamples | SAMEA104130970 |
Cellosaurus | CVCL_VF11 |
CLO | CLO_0101660 |
Wikidata | Q54989674 |
General Information |
|
* Is the cell line readily obtainable for third parties? |
Yes Research use: allowed
Clinical use: not allowed
Commercial use: allowed
|
Donor Information#
General Donor Information |
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Sex | male |
Phenotype and Disease related information (Donor) |
|
Diseases | A disease was diagnosed.
|
External Databases (Donor) |
|
BioSamples | SAMEA104130971 |
hIPSC Derivation#
General |
|
Source cell type |
fibroblast of dermis |
Source cell origin |
zone of skinAny portion of the organ that covers that body and consists of a layer of epidermis and a layer of dermis.
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Reprogramming method |
|
Vector type | Integrating |
Vector | Virus (Lentivirus) |
Genes | |
Is the used vector excisable? |
No |
Absence of reprogramming vector(s)? |
No |
Reprogramming vectors silenced? |
Yes |
Methods used |
Immunostaining, RT-PCR
|
Vector map | |
Vector free reprogramming |
|
Other |
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Derived under xeno-free conditions |
Unknown |
Derived under GMP? |
Unknown |
Available as clinical grade? |
Unknown |
Culture Conditions#
The following are the depositor culture conditions, they do not refer to any specific batch.
Surface coating | Vitronectin |
Feeder cells |
No |
Passage method |
Enzyme-free cell dissociation
EDTA
|
Medium |
TeSR™ E8™
|
Characterisation#
Analysis of Undifferentiated Cells
Marker | Expressed | Immunostaining | RT-PCR | FACS | Enzymatic Assay | Expression Profiles |
NANOG |
Yes |
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SOX2 |
Yes |
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POU5F1 (OCT-4) |
Yes |
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Alkaline Phosphatase |
Yes |
Genotyping#
Karyotyping (Cell Line) |
|
Has the cell line karyotype been analysed? |
Yes
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Other Genotyping (Cell Line) |