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UCLi005-A

LGMD2D Pt. 4, LGMD2D #4

iPSC line

The cell line is not available.

General#

Cell Line

hPSCreg Name UCLi005-A
Alternative name(s)
LGMD2D Pt. 4, LGMD2D #4
Cell line type Human induced pluripotent stem cell (hiPSC)

Provider

Depositor University College London (UCL)
Owner Dept of Cell and Developmental Biology
Distributors
Dept of Cell and Developmental Biology
EBiSC
European Collection of Authenticated Cell Cultures (ECACC)

External Databases

hPSCreg UCLi005-A
Cellosaurus CVCL_9S57
BioSamples SAMEA4091959
CLO CLO_0101571
Wikidata Q54989673

General Information

Publications
* Is the cell line readily obtainable for third parties?
Yes
Research use: allowed
Clinical use: not allowed
Commercial use: not allowed

Donor Information#

General Donor Information

Sex male
Age of donor (at collection) 10-14

Phenotype and Disease related information (Donor)

Diseases A disease was diagnosed.
limb-girdle muscular dystrophy
LGMD 2D
The donor is affected.
Genetic variants
SGCA (target)
17q21.33
See publication Supplemental Data table S1: Homozygote, exon 3, 229 C>T, Arg77Cys
Disease associated phenotypes
  • Skeletal muscle weakness, wasting and dystrophy
Non-disease associated phenotypes
  • LGMD2D

Karyotyping (Donor)

Has the donor karyotype been analysed?
Unknown

External Databases (Donor)

BioSamples SAMEA4091958

hIPSC Derivation#

General

Source cell type
skeletal muscle myoblast
A myoblast that differentiates into skeletal muscle fibers.
Age of donor (at collection) 10-14

Reprogramming method

Vector type Integrating
Vector Virus (Retrovirus)
Is the used vector excisable?
No
Absence of reprogramming vector(s)?
No
Reprogramming vectors silenced?

Vector free reprogramming

Other

Derived under xeno-free conditions
No
Derived under GMP?
No
Available as clinical grade?
No

Culture Conditions#

The following are the depositor culture conditions, they do not refer to any specific batch.
Surface coating Matrigel/Geltrex
Feeder cells
No
O2 Concentration 5 %
Medium TeSR™ E8™

Characterisation#

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR FACS Enzymatic Assay Expression Profiles
Alkaline Phosphatase
Yes
SOX2
Yes
POU5F1 (OCT-4)
Yes
Morphology pictures
eb71c48cca238458a3e2dd9cf18f8c57.pdf
Morphology of undifferentiated LGMD2D iPSCs #4 grown without feeder layer cells in chemically defined media. Approximate passage number: 10.
Differentiation Potency
Endoderm
Ont Id: UBERON_0000925
In vitro spontaneous differentiation
Marker Expressed
AFP
Yes
GATA4
Yes
FOXA2
Yes
Cell Of Skeletal Muscle
Ont Id: CL_0000188
In vitro spontaneous differentiation
In vitro directed differentiation
Marker Expressed
MSX1
Yes
Ectoderm
Ont Id: UBERON_0000924
In vitro spontaneous differentiation
Marker Expressed
MAP2
Yes
PAX6
Yes

Genotyping#

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
Yes
LGMD2D Pt.4 HIDEMs (differentiated iPSCs)
Karyotyping method: G-Banding

Other Genotyping (Cell Line)