UCLi005-A
LGMD2D Pt. 4, LGMD2D #4
iPSC line
The cell line is not available.
General#
Cell Line |
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hPSCreg Name | UCLi005-A |
Alternative name(s) |
LGMD2D Pt. 4, LGMD2D #4
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Cell line type | Human induced pluripotent stem cell (hiPSC) |
Provider |
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Depositor | University College London (UCL) |
Owner | Dept of Cell and Developmental Biology |
Distributors |
Dept of Cell and Developmental Biology
EBiSC
European Collection of Authenticated Cell Cultures (ECACC)
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External Databases |
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hPSCreg | UCLi005-A |
Cellosaurus | CVCL_9S57 |
BioSamples | SAMEA4091959 |
CLO | CLO_0101571 |
Wikidata | Q54989673 |
General Information |
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Publications | |
* Is the cell line readily obtainable for third parties? |
Yes Research use: allowed
Clinical use: not allowed
Commercial use: not allowed
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Donor Information#
General Donor Information |
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Sex | male |
Age of donor (at collection) | 10-14 |
Phenotype and Disease related information (Donor) |
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Diseases | A disease was diagnosed.
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Disease associated phenotypes |
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Non-disease associated phenotypes |
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Karyotyping (Donor) |
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Has the donor karyotype been analysed? |
Unknown
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External Databases (Donor) |
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BioSamples | SAMEA4091958 |
hIPSC Derivation#
General |
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Source cell type |
skeletal muscle myoblastA myoblast that differentiates into skeletal muscle fibers.
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Age of donor (at collection) | 10-14 |
Reprogramming method |
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Vector type | Integrating |
Vector | Virus (Retrovirus) |
Is the used vector excisable? |
No |
Absence of reprogramming vector(s)? |
No |
Reprogramming vectors silenced? | |
Vector free reprogramming |
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Other |
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Derived under xeno-free conditions |
No |
Derived under GMP? |
No |
Available as clinical grade? |
No |
Culture Conditions#
The following are the depositor culture conditions, they do not refer to any specific batch.
Surface coating | Matrigel/Geltrex |
Feeder cells |
No |
O2 Concentration | 5 % |
Medium |
TeSR™ E8™
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Characterisation#
Analysis of Undifferentiated Cells
Marker | Expressed | Immunostaining | RT-PCR | FACS | Enzymatic Assay | Expression Profiles |
Alkaline Phosphatase |
Yes |
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SOX2 |
Yes |
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POU5F1 (OCT-4) |
Yes |
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Morphology pictures
eb71c48cca238458a3e2dd9cf18f8c57.pdf
Morphology of undifferentiated LGMD2D iPSCs #4 grown without feeder layer cells in chemically defined media. Approximate passage number: 10.
Differentiation Potency
In vitro spontaneous differentiation
In vitro directed differentiation
Marker | Expressed |
MSX1 |
Yes |
In vitro spontaneous differentiation
Marker | Expressed |
MAP2 |
Yes |
PAX6 |
Yes |
Genotyping#
Karyotyping (Cell Line) |
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Has the cell line karyotype been analysed? |
Yes
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Other Genotyping (Cell Line) |