UCLi005-A
LGMD2D Pt. 4, LGMD2D #4
iPSC line
The cell line is not available.
General#
Cell Line |
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hPSCreg Name | UCLi005-A |
Alternative name(s) |
LGMD2D Pt. 4, LGMD2D #4
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Cell line type | Human induced pluripotent stem cell (hiPSC) |
Similar lines |
CENSOi003-B (FB79R2c6, CENSOi258) Donor's gene variants: DMD, DMD Donor diseases: Duchenne muscular dystrophy CENSOi005-A (FB76R2c5, CENSOi245) Donor's gene variants: DMD, DMD Donor diseases: Duchenne muscular dystrophy CENSOi007-A (FB75R2c5, CENSOi255) Donor's gene variants: DMD, DMD Donor diseases: Duchenne muscular dystrophy CENSOi001-B (FB78R2c2, CENSOi249) Donor's gene variants: DMD, DMD Donor diseases: Duchenne muscular dystrophy CENSOi002-B (FB74R2c4, CENSOi261) Donor's gene variants: DMD, DMD Donor diseases: Duchenne muscular dystrophy RCi006-A (FB69c4, RCi169) Donor's gene variants: LRIF1, LRIF1 Donor diseases: Facioscapulohumeral dystrophy RCi009-A (Rci201, FB73c6) Donor's gene variants: LRIF1, LRIF1 Donor diseases: Facioscapulohumeral dystrophy CENSOi008-A (FB77R2c3, CENSOi260) Donor's gene variants: DMPK, DMPK Donor diseases: myotonic dystrophy type 1 RCi005-A (FB71c4, RCi171) Donor's gene variants: LRIF1, LRIF1 Donor diseases: Facioscapulohumeral dystrophy UCLi002-A (HHItC9D-V34, DN19) Donor's gene variants: C9orf72, C9orf72 Donor diseases: Frontotemporal dementia UCLi004-B (RCFB60c7, RCi177) Donor's gene variants: C9orf72, C9orf72 Donor diseases: Amyotrophic lateral sclerosis Frontotemporal dementia UCLi004-C (RCi172, RCFB60c2) Donor's gene variants: C9orf72, C9orf72 Donor diseases: Amyotrophic lateral sclerosis Frontotemporal dementia LUBi001-B (PGRN-8310, RCFB58 c4.4, PGRN8310, RCi194) Donor's gene variants: GRN, GRN, GRN, GRN Donor diseases: Frontotemporal dementia LUBi001-C (PGRN-8310, RCFB58 c3.7, RCi200, PGRN8310) Donor's gene variants: GRN, GRN, GRN, GRN Donor diseases: Frontotemporal dementia UNEWi026-A (SF116 clone 1) Donor's gene variants: CFH Donor diseases: type 2 diabetes mellitus age-related macular degeneration UNEWi026-B (SF116 clone 2) Donor's gene variants: CFH Donor diseases: type 2 diabetes mellitus age-related macular degeneration UNEWi026-C (SF116 clone K) Donor's gene variants: CFH Donor diseases: type 2 diabetes mellitus age-related macular degeneration |
Provider |
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Depositor | University College London (UCL) |
Owner | Dept of Cell and Developmental Biology |
Distributors |
Dept of Cell and Developmental Biology
EBiSC
European Collection of Authenticated Cell Cultures (ECACC)
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External Databases |
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hPSCreg | UCLi005-A |
Cellosaurus | CVCL_9S57 |
BioSamples | SAMEA4091959 |
CLO | CLO_0101571 |
Wikidata | Q54989673 |
General Information |
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Publications | |
* Is the cell line readily obtainable for third parties? |
Yes Research use: allowed
Clinical use: not allowed
Commercial use: not allowed
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Donor Information#
General Donor Information |
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Sex | male |
Age of donor (at collection) | 10-14 |
Phenotype and Disease related information (Donor) |
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Diseases | A disease was diagnosed.
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Disease associated phenotypes |
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Non-disease associated phenotypes |
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Karyotyping (Donor) |
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Has the donor karyotype been analysed? |
Unknown
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External Databases (Donor) |
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BioSamples | SAMEA4091958 |
hIPSC Derivation#
General |
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Source cell type |
skeletal muscle myoblastA myoblast that differentiates into skeletal muscle fibers.
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Age of donor (at collection) | 10-14 |
Reprogramming method |
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Vector type | Integrating |
Vector | Virus (Retrovirus) |
Is the used vector excisable? |
No |
Absence of reprogramming vector(s)? |
No |
Reprogramming vectors silenced? | |
Vector free reprogramming |
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Other |
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Derived under xeno-free conditions |
No |
Derived under GMP? |
No |
Available as clinical grade? |
No |
Culture Conditions#
The following are the depositor culture conditions, they do not refer to any specific batch.
Surface coating | Matrigel/Geltrex |
Feeder cells |
No |
O2 Concentration | 5 % |
Medium |
TeSR™ E8™
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Characterisation#
Analysis of Undifferentiated Cells
Marker | Expressed | Immunostaining | RT-PCR | FACS | Enzymatic Assay | Expression Profiles |
Alkaline Phosphatase |
Yes |
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SOX2 |
Yes |
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POU5F1 (OCT-4) |
Yes |
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Morphology pictures
eb71c48cca238458a3e2dd9cf18f8c57.pdf
Morphology of undifferentiated LGMD2D iPSCs #4 grown without feeder layer cells in chemically defined media. Approximate passage number: 10.
Differentiation Potency
In vitro spontaneous differentiation
In vitro directed differentiation
Marker | Expressed |
MSX1 |
Yes |
In vitro spontaneous differentiation
Marker | Expressed |
MAP2 |
Yes |
PAX6 |
Yes |
Genotyping#
Karyotyping (Cell Line) |
|
Has the cell line karyotype been analysed? |
Yes
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Other Genotyping (Cell Line) |