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UCLi005-A
LGMD2D Pt. 4, LGMD2D #4
iPSC line
The cell line is not released.
General#
Cell Line |
|
| hPSCreg name | UCLi005-A |
| Alternative name(s) |
LGMD2D Pt. 4, LGMD2D #4
|
| Cell line type | Human induced pluripotent stem cell (hiPSC) |
| Similar lines | No similar lines found. |
Provider |
|
| Depositor | University College London (UCL) |
| Owner | Dept of Cell and Developmental Biology |
| Distributors |
Dept of Cell and Developmental Biology
EBiSC
|
External Databases |
|
| hPSCreg | UCLi005-A |
| Cellosaurus | CVCL_9S57 |
| BioSamples | SAMEA4091959 |
| Wikidata | Q54989673 |
General Information |
|
| Publications | |
| This EBiSC line can be used for: |
Yes
Research use: allowed
Clinical use: no
Commercial use: no
|
Donor Information#
General Donor Information |
|
| Sex | male |
| Age of donor (at collection) | 10-14 |
Phenotype and Disease related information (Donor) |
|
| Diseases | A disease was diagnosed.
|
| Disease associated phenotypes |
|
| Non-disease associated phenotypes |
|
Karyotyping (Donor) |
|
| Has the donor karyotype been analysed? |
Unknown
|
External Databases (Donor) |
|
| BioSamples | SAMEA4091958 |
hIPSC Derivation#
General |
|
| Source cell type |
A myoblast that differentiates into skeletal muscle fibers.
Synonyms
|
| Age of donor (at collection) | 10-14 |
Reprogramming method |
|
| Vector type | Integrating |
| Vector | Virus (Retrovirus) |
| Is the used vector excisable? |
No |
| Absence of reprogramming vector(s)? |
No |
| Reprogramming vectors silenced? | |
Vector free reprogramming |
|
Other |
|
| Derived under xeno-free conditions |
No |
| Derived under GMP? |
No |
| Available as clinical grade? |
No |
Culture Conditions#
The following are the depositor culture conditions, they do not refer to any specific batch.
| Surface coating | Matrigel/Geltrex |
| Feeder cells |
No |
| O2 Concentration | 5 % |
| Medium |
TeSR™ E8™
|
Characterisation#
Analysis of Undifferentiated Cells
| Marker | Expressed | Immunostaining | RT-PCR | Flow Cytometry | Enzymatic Assay | Expression Profiles |
| Alkaline Phosphatase |
Yes |
|
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| SOX2 |
Yes |
|
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| POU5F1 (OCT-4) |
Yes |
|
Morphology pictures
eb71c48cca238458a3e2dd9cf18f8c57.pdf
Morphology of undifferentiated LGMD2D iPSCs #4 grown without feeder layer cells in chemically defined media. Approximate passage number: 10.
Differentiation Potency
In vitro spontaneous differentiation
In vitro directed differentiation
| Marker | Expressed |
| MSX1 |
Yes |
In vitro spontaneous differentiation
| Marker | Expressed |
| MAP2 |
Yes |
| PAX6 |
Yes |
Genotyping#
Karyotyping (Cell Line) |
|
| Has the cell line karyotype been analysed? |
Yes
|
Other Genotyping (Cell Line) |
|