UCLi005-A
LGMD2D Pt. 4, LGMD2D #4
iPSC line
The cell line is not available.
General#
Cell Line |
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| hPSCreg Name | UCLi005-A |
| Alternative name(s) |
LGMD2D Pt. 4, LGMD2D #4
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| Cell line type | Human induced pluripotent stem cell (hiPSC) |
Provider |
|
| Depositor | University College London (UCL) |
| Owner | Dept of Cell and Developmental Biology |
| Distributors |
Dept of Cell and Developmental Biology
EBiSC
European Collection of Authenticated Cell Cultures (ECACC)
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External Databases |
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| hPSCreg | UCLi005-A |
| Cellosaurus | CVCL_9S57 |
| BioSamples | SAMEA4091959 |
| CLO | CLO_0101571 |
| Wikidata | Q54989673 |
General Information |
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| Publications | |
| * Is the cell line readily obtainable for third parties? |
Yes Research use: allowed
Clinical use: not allowed
Commercial use: not allowed
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Donor Information#
General Donor Information |
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| Sex | male |
| Age of donor (at collection) | 10-14 |
Phenotype and Disease related information (Donor) |
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| Diseases | A disease was diagnosed.
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| Disease associated phenotypes |
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| Non-disease associated phenotypes |
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Karyotyping (Donor) |
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| Has the donor karyotype been analysed? |
Unknown
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External Databases (Donor) |
|
| BioSamples | SAMEA4091958 |
hIPSC Derivation#
General |
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| Source cell type |
skeletal muscle myoblastA myoblast that differentiates into skeletal muscle fibers.
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| Age of donor (at collection) | 10-14 |
Reprogramming method |
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| Vector type | Integrating |
| Vector | Virus (Retrovirus) |
| Is the used vector excisable? |
No |
| Absence of reprogramming vector(s)? |
No |
| Reprogramming vectors silenced? | |
Vector free reprogramming |
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Other |
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| Derived under xeno-free conditions |
No |
| Derived under GMP? |
No |
| Available as clinical grade? |
No |
Culture Conditions#
The following are the depositor culture conditions, they do not refer to any specific batch.
| Surface coating | Matrigel/Geltrex |
| Feeder cells |
No |
| O2 Concentration | 5 % |
| Medium |
TeSR™ E8™
|
Characterisation#
Analysis of Undifferentiated Cells
| Marker | Expressed | Immunostaining | RT-PCR | FACS | Enzymatic Assay | Expression Profiles |
| Alkaline Phosphatase |
Yes |
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| SOX2 |
Yes |
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| POU5F1 (OCT-4) |
Yes |
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Morphology pictures
eb71c48cca238458a3e2dd9cf18f8c57.pdf
Morphology of undifferentiated LGMD2D iPSCs #4 grown without feeder layer cells in chemically defined media. Approximate passage number: 10.
Differentiation Potency
In vitro spontaneous differentiation
In vitro directed differentiation
| Marker | Expressed |
| MSX1 |
Yes |
In vitro spontaneous differentiation
| Marker | Expressed |
| MAP2 |
Yes |
| PAX6 |
Yes |
Genotyping#
Karyotyping (Cell Line) |
|
| Has the cell line karyotype been analysed? |
Yes
|
Other Genotyping (Cell Line) |
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