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STBCi093-A

SFC893-07-06

iPSC line

Not-for-profit fee: £1400 per vial
Not released.
The cell line is not released.

General#

Cell Line

hPSCreg name STBCi093-A
Alternative name(s)
SFC893-07-06
Cell line type Human induced pluripotent stem cell (hiPSC)
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Provider

Depositor StemBANCC (STBC)
Distributors
EBiSC

External Databases

hPSCreg STBCi093-A
BioSamples SAMEA104493897
Cellosaurus CVCL_RD55
Wikidata Q54970224

General Information

This EBiSC line can be used for:
Yes
Research use: allowed
Clinical use: no
Commercial use: no

Donor Information#

General Donor Information

Sex male
Age of donor (at collection) 65-69

Phenotype and Disease related information (Donor)

Diseases A disease was diagnosed.
Synonyms
  • Diabetes mellitus (disorder)
  • Diabetes
  • Diabetes mellitus, NOS
  • DM - Diabetes mellitus
  • Diabetes NOS
  • diabetes mellitus (disease)
  • DM
  • diabetes mellitus
  • diabetes
show more synonyms
Genetic variants
Wolfram

Donor Relations

Other cell lines of this donor

External Databases (Donor)

BioSamples SAMEA104493898

hIPSC Derivation#

General

Source cell type
A connective tissue cell which secretes an extracellular matrix rich in collagen and other macromolecules. Flattened and irregular in outline with branching processes; appear fusiform or spindle-shaped.; These cells may be vimentin-positive, fibronectin-positive, fsp1-positive, MMP-1-positive, collagen I-positive, collagen III-positive, and alpha-SMA-negative.
Age of donor (at collection) 65-69

Reprogramming method

Vector type Non-integrating
Vector Sendai virus

Vector free reprogramming

Other

Derived under xeno-free conditions
Unknown
Derived under GMP?
Unknown
Available as clinical grade?
Unknown

Culture Conditions#

The following are the depositor culture conditions, they do not refer to any specific batch.
Surface coating Matrigel/Geltrex
Feeder cells
No
Passage method Enzyme-free cell dissociation
EDTA
O2 Concentration 21 %
CO2 Concentration 5 %
Medium mTeSR™ 1

Characterisation#

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR Flow Cytometry Enzymatic Assay Expression Profiles
NANOG
Yes
TRA 1-60
Yes

Microbiology / Virus Screening

Mycoplasma Negative

Genotyping#

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
Yes
minor Chr20 abnormality which is not present in parental fibroblasts
Passage number: 16
Karyotyping method: Molecular karyotyping by SNP array
http://

Other Genotyping (Cell Line)