STBCi093-A
SFC893-07-06
iPSC line
The cell line is not available.
General#
Cell Line |
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| hPSCreg Name | STBCi093-A |
| Alternative name(s) |
SFC893-07-06
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| Cell line type | Human induced pluripotent stem cell (hiPSC) |
Provider |
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| Depositor | StemBANCC (STBC) |
| Distributors |
EBiSC
European Collection of Authenticated Cell Cultures (ECACC)
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External Databases |
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| hPSCreg | STBCi093-A |
| BioSamples | SAMEA104493897 |
| Cellosaurus | CVCL_RD55 |
| CLO | CLO_0101916 |
| Wikidata | Q54970224 |
General Information |
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| * Is the cell line readily obtainable for third parties? |
Yes Research use: allowed
Clinical use: not allowed
Commercial use: not allowed
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Donor Information#
General Donor Information |
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| Sex | male |
| Age of donor (at collection) | 65-69 |
Phenotype and Disease related information (Donor) |
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| Diseases | A disease was diagnosed.
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Donor Relations |
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| Other cell lines of this donor | |
External Databases (Donor) |
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| BioSamples | SAMEA104493898 |
hIPSC Derivation#
General |
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| Source cell type |
fibroblastA connective tissue cell which secretes an extracellular matrix rich in collagen and other macromolecules. Flattened and irregular in outline with branching processes; appear fusiform or spindle-shaped.
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| Age of donor (at collection) | 65-69 |
Reprogramming method |
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| Vector type | Non-integrating |
| Vector | Sendai virus |
Vector free reprogramming |
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Other |
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| Derived under xeno-free conditions |
Unknown |
| Derived under GMP? |
Unknown |
| Available as clinical grade? |
Unknown |
Culture Conditions#
The following are the depositor culture conditions, they do not refer to any specific batch.
| Surface coating | Matrigel/Geltrex |
| Feeder cells |
No |
| Passage method |
Enzyme-free cell dissociation
EDTA
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| O2 Concentration | 21 % |
| CO2 Concentration | 5 % |
| Medium |
mTeSR™ 1
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Characterisation#
Analysis of Undifferentiated Cells
| Marker | Expressed | Immunostaining | RT-PCR | FACS | Enzymatic Assay | Expression Profiles |
| NANOG |
Yes |
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| TRA 1-60 |
Yes |
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Microbiology / Virus Screening |
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| Mycoplasma | Negative |
Genotyping#
Karyotyping (Cell Line) |
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| Has the cell line karyotype been analysed? |
Yes
minor Chr20 abnormality which is not present in parental fibroblasts
Passage number: 16
Karyotyping method:
Molecular karyotyping by SNP array
http:// |
Other Genotyping (Cell Line) |
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