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MRIi028-A

PPC-4

iPSC line

The cell line is not available.

General#

Cell Line
hPSCreg Name MRIi028-A
Alternative name(s)
PPC-4
Cell line type Human induced pluripotent stem cell (hiPSC)
Similar lines

Provider
Depositor Klinikum rechts der Isar (MRI)
Distributors
European Collection of Authenticated Cell Cultures (ECACC)

External Databases
hPSCreg MRIi028-A
BioSamples SAMEA9589929
Cellosaurus CVCL_A0TH
CLO CLO_0103618
Wikidata Q108820976

General Information
Publications View all related publications on hPSCreg (3)
* Is the cell line readily obtainable for third parties?
Yes
Research use: allowed
Clinical use: not allowed
Commercial use: not allowed

Donor Information#

General Donor Information
Sex male

Phenotype and Disease related information (Donor)
Diseases A disease was diagnosed.
PPCS-deficiency OMIM *609853
The donor is a carrier of a disease-associated mutation and affected.
Disease associated phenotypes
  • CARDIOMYOPATHY, DILATED, 2C, WITH MUSCULAR HYPOTONIA AND DYSMORPHIC FEATURES
Is the medical history available upon request? YES
Is clinical information available? YES

Karyotyping (Donor)
Has the donor karyotype been analysed?
Yes
46, XY
kariotyping_PPCS-4.pdf
Karyotyping method: Spectral karyotyping

Other Genotyping (Donor)
Is there genome-wide genotyping or functional data available?
No

External Databases (Donor)
BioSamples SAMEA9589930

hIPSC Derivation#

General
Source cell type
Fibroblasts
Fibroblasts in the OLS
Source cell origin
Skin
Skin in the OLS
An organ that constitutes the external surface of the body. It consists of the epidermis, dermis, and skin appendages.
Synonyms
  • Skin
  • Human Skin
  • SKIN
  • Integument
  • Skin, total
  • Skin, NOS
show more synonyms

Reprogramming method
Vector type Non-integrating
Vector Sendai virus
Is reprogramming vector detectable?
No
Methods used
RT-PCR
Files and images showing reprogramming vector expressed or silenced
Sendai-Virus_PCR.pdf

Vector free reprogramming

Other
Derived under xeno-free conditions
Unknown
Derived under GMP?
Unknown
Available as clinical grade?
Unknown

Culture Conditions#

The following are the depositor culture conditions, they do not refer to any specific batch.
Surface coating Matrigel/Geltrex
Feeder cells
No
Passage method Enzymatically
Dispase
CO2 Concentration 5 %
Medium Essential 8™
Supplements
E8 supplement 10 ml
Pen/Strep 2,5 ml
Has Rock inhibitor (Y27632) been used at passage previously with this cell line?
No
Has Rock inhibitor (Y27632) been used at cryo previously with this cell line?
No
Has Rock inhibitor (Y27632) been used at thaw previously with this cell line?
No

Characterisation#

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR FACS Enzymatic Assay Expression Profiles
TRA 1-81
Yes
–
NANOG
Yes
–
Differentiation Potency
Endoderm
Ont Id: UBERON_0000925
In vitro spontaneous differentiation
Marker Expressed
SOX7 SRY-box transcription factor 7
Unknown
Protocol or reference
germlayer PPC-4.pdf
Mesoderm
Ont Id: UBERON_0000926
In vitro spontaneous differentiation
Marker Expressed
ACTA2 – actin alpha 2, smooth muscle
Unknown
Protocol or reference
germlayer PPC-4.pdf
Ectoderm
Ont Id: UBERON_0000924
In vitro spontaneous differentiation
Marker Expressed
KRT14 keratin 14
Unknown
Protocol or reference
germlayer PPC-4.pdf

Genotyping#

Karyotyping (Cell Line)
Has the cell line karyotype been analysed?
Unknown

Other Genotyping (Cell Line)