MRIi028-A
PPC-4
iPSC line
The cell line is not available.
General#
Cell Line |
|
hPSCreg Name | MRIi028-A |
Alternative name(s) |
PPC-4
|
Cell line type | Human induced pluripotent stem cell (hiPSC) |
Similar lines | |
Provider |
|
Depositor | Klinikum rechts der Isar (MRI) |
Distributors |
European Collection of Authenticated Cell Cultures (ECACC)
|
External Databases |
|
hPSCreg | MRIi028-A |
BioSamples | SAMEA9589929 |
Cellosaurus | CVCL_A0TH |
CLO | CLO_0103618 |
Wikidata | Q108820976 |
General Information |
|
Publications | View all related publications on hPSCreg (3) |
* Is the cell line readily obtainable for third parties? |
Yes Research use: allowed
Clinical use: not allowed
Commercial use: not allowed
|
Donor Information#
General Donor Information |
|
Sex | male |
Phenotype and Disease related information (Donor) |
|
Diseases | A disease was diagnosed.
|
Disease associated phenotypes |
|
Is the medical history available upon request? | YES |
Is clinical information available? | YES |
Karyotyping (Donor) |
|
Has the donor karyotype been analysed? |
Yes
|
Other Genotyping (Donor) |
|
Is there genome-wide genotyping or functional data available? |
No
|
External Databases (Donor) |
|
BioSamples | SAMEA9589930 |
hIPSC Derivation#
General |
|
Source cell type |
Fibroblasts |
Source cell origin |
SkinAn organ that constitutes the external surface of the body. It consists of the epidermis, dermis, and skin appendages.
Synonyms
|
Reprogramming method |
|
Vector type | Non-integrating |
Vector | Sendai virus |
Is reprogramming vector detectable? |
No |
Methods used |
RT-PCR
|
Files and images showing reprogramming vector expressed or silenced | |
Vector free reprogramming |
|
Other |
|
Derived under xeno-free conditions |
Unknown |
Derived under GMP? |
Unknown |
Available as clinical grade? |
Unknown |
Culture Conditions#
The following are the depositor culture conditions, they do not refer to any specific batch.
Surface coating | Matrigel/Geltrex | |||||||||
Feeder cells |
No |
|||||||||
Passage method |
Enzymatically
Dispase
|
|||||||||
CO2 Concentration | 5 % | |||||||||
Medium |
Essential 8™
Supplements
|
|||||||||
Has Rock inhibitor (Y27632) been used at passage previously with this cell line? | No |
|||||||||
Has Rock inhibitor (Y27632) been used at cryo previously with this cell line? | No |
|||||||||
Has Rock inhibitor (Y27632) been used at thaw previously with this cell line? | No |
Characterisation#
Analysis of Undifferentiated Cells
Marker | Expressed | Immunostaining | RT-PCR | FACS | Enzymatic Assay | Expression Profiles |
TRA 1-81 |
Yes |
|||||
NANOG |
Yes |
Differentiation Potency
In vitro spontaneous differentiation
Marker | Expressed |
SOX7 SRY-box transcription factor 7 |
Unknown |
Protocol or reference
In vitro spontaneous differentiation
Marker | Expressed |
ACTA2 – actin alpha 2, smooth muscle |
Unknown |
Protocol or reference
In vitro spontaneous differentiation
Marker | Expressed |
KRT14 keratin 14 |
Unknown |
Protocol or reference
Genotyping#
Karyotyping (Cell Line) |
|
Has the cell line karyotype been analysed? |
Unknown
|
Other Genotyping (Cell Line) |