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LBi018-A

PH-BL-409 Clone 43, iLB-291bfs43

iPSC line

The cell line is not available.

General#

Cell Line
hPSCreg Name LBi018-A
Alternative name(s)
PH-BL-409 Clone 43, iLB-291bfs43
Cell line type Human induced pluripotent stem cell (hiPSC)

Provider
Depositor Life & Brain GmbH (LB)
Distributors
EBiSC
European Collection of Authenticated Cell Cultures (ECACC)
Derivation country Germany

External Databases
hPSCreg LBi018-A
BioSamples SAMEA111531217
CLO CLO_0104414
Cellosaurus CVCL_C6RF

General Information
* Is the cell line readily obtainable for third parties?
Yes
Research use: allowed
Clinical use: allowed
Commercial use: not allowed

Donor Information#

General Donor Information
Sex female
Age of donor (at collection) 55-59
Ethnicity White European

Phenotype and Disease related information (Donor)
Diseases A disease was diagnosed.
Alzheimer disease
Alzheimer disease in the OLS
TREM2R47H/WT CD33 full length variant /full length variant
The donor is a carrier of a disease-associated mutation and not affected.
Stage
Cognitively normal on the day of sample donation
Synonyms
  • Late-onset form of familial Alzheimer disease
  • Alzheimer disease
Genetic variants
TREM2 (target)
6p21.1
NG_011561.1:g.6671G>A
Heterozygous

Karyotyping (Donor)
Has the donor karyotype been analysed?
Unknown

Donor Relations
Other cell lines of this donor

External Databases (Donor)
BioSamples SAMEA111531224

hIPSC Derivation#

General
Source cell type
erythroid progenitor cell
erythroid progenitor cell in the OLS
Committed, erythroid stem cells derived from myeloid stem cells. The progenitor cells develop in two phases: erythroid burst-forming units (bfu-e) followed by erythroid colony-forming units (cfu-e). Bfu-e differentiate into cfu-e on stimulation by erythropoietin, and then further differentiate into erythroblasts when stimulated by other factors.
Source cell origin
Blood
Blood in the OLS
A liquid tissue; its major function is to transport oxygen throughout the body. It also supplies the tissues with nutrients, removes waste products, and contains various components of the immune system defending the body against infection. Several hormones also travel in the blood.
Synonyms
  • Reticuloendothelial System, Blood
  • Peripheral Blood
  • peripheral blood
  • blood
  • Blood
  • Whole Blood
  • BLOOD
show more synonyms
Age of donor (at collection) 55-59
Collected in 2018

Reprogramming method
Vector type Non-integrating
Vector Sendai virus
Is reprogramming vector detectable?
No
Methods used
RT-PCR
Files and images showing reprogramming vector expressed or silenced
LBi018-A_TRA-1-60.png

Vector free reprogramming

Other
Derived under xeno-free conditions
No
Derived under GMP?
No
Available as clinical grade?
No

Culture Conditions#

The following are the depositor culture conditions, they do not refer to any specific batch.
Surface coating Vitronectin
Feeder cells
No
Passage method Enzyme-free cell dissociation
EDTA
O2 Concentration 20 %
CO2 Concentration 6 %
Medium Other medium:
Base medium: StemMACS™ iPS-Brew XF, Basal Medium
Main protein source: StemMACS™ iPS Brew XF, 50x Supplement
Serum concentration: %
Has Rock inhibitor (Y27632) been used at passage previously with this cell line?
Yes
Has Rock inhibitor (Y27632) been used at cryo previously with this cell line?
No
Has Rock inhibitor (Y27632) been used at thaw previously with this cell line?
Yes

Characterisation#

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR FACS Enzymatic Assay Expression Profiles
TRA 1-60
Yes
–
Morphology pictures

Genotyping#

Karyotyping (Cell Line)
Has the cell line karyotype been analysed?
Yes
Normal 46XX
Karyotyping method: Molecular karyotyping by SNP array
http://
LBi018-A_SNP_array.png

Other Genotyping (Cell Line)