The EBiSC team is working hard to implement improvements in how EBiSC operates. Due to some short-term disruption, please get in touch via if the cells you would like to access are currently listed as unavailable or you are ordering from outside of Europe.


PH-BL-304 Clone 117, iLB-279bfs117

iPSC line

Not released.
We are currently making some changes to how EBiSC operates and because of this there is a short period of time where orders cannot be placed.

If you are interested in accessing these cells, please contact EBiSC directly. For more information about the current transition process see here.
The cell line is not released.


Cell Line

hPSCreg name LBi013-A
Alternative name(s)
PH-BL-304 Clone 117, iLB-279bfs117
Cell line type Human induced pluripotent stem cell (hiPSC)
Similar lines No similar lines found.
Notes CD33 high risk Alzheimer disease TREM2-/- CD33+/+ (TREM2WT/WT, CD33M/M)


Depositor Life & Brain GmbH (LB)
Derivation country Germany

External Databases

hPSCreg LBi013-A
BioSamples SAMEA111521229
Cellosaurus CVCL_C6R6
Wikidata Q117704618

General Information

This EBiSC line can be used for:
Research use: allowed
Clinical use: no
Commercial use: no

Donor Information#

General Donor Information

Sex female
Age of donor (at collection) 70-74
Ethnicity White European

Phenotype and Disease related information (Donor)

Diseases A disease was diagnosed.
TREM2WT/WT CD33 full length variant /full length variant
The donor is not a carrier of a disease-associated mutation and not affected.
Cognitively normal on the day of sample donation
  • Late-onset form of familial Alzheimer disease
  • Alzheimer disease
Disease associated phenotypes no phenotypes

Karyotyping (Donor)

Has the donor karyotype been analysed?

External Databases (Donor)

BioSamples SAMEA111522583

hIPSC Derivation#


Source cell type
Committed, erythroid stem cells derived from myeloid stem cells. The progenitor cells develop in two phases: erythroid burst-forming units (bfu-e) followed by erythroid colony-forming units (cfu-e). Bfu-e differentiate into cfu-e on stimulation by erythropoietin, and then further differentiate into erythroblasts when stimulated by other factors.
Source cell origin
A liquid tissue; its major function is to transport oxygen throughout the body. It also supplies the tissues with nutrients, removes waste products, and contains various components of the immune system defending the body against infection. Several hormones also travel in the blood.
  • Reticuloendothelial System, Blood
  • Peripheral Blood
  • peripheral blood
  • blood
  • Blood
  • Whole Blood
  • portion of blood
  • vertebrate blood
show more synonyms
Age of donor (at collection) 70-74
Collected in 2018

Reprogramming method

Vector type Non-integrating
Vector Sendai virus
Is reprogramming vector detectable?
Methods used
Files and images showing reprogramming vector expressed or silenced

Vector free reprogramming


Derived under xeno-free conditions
Derived under GMP?
Available as clinical grade?

Culture Conditions#

The following are the depositor culture conditions, they do not refer to any specific batch.
Surface coating Vitronectin
Feeder cells
Passage method Enzymatically
O2 Concentration 20 %
CO2 Concentration 6 %
Medium Other medium:
Base medium: StemMACS™ iPS-Brew XF, Basal Medium
Main protein source: StemMACS™ iPS Brew XF, 50x Supplement
Serum concentration: %
Has Rock inhibitor (Y27632) been used at passage previously with this cell line?
Has Rock inhibitor (Y27632) been used at cryo previously with this cell line?
Has Rock inhibitor (Y27632) been used at thaw previously with this cell line?


Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR Flow Cytometry Enzymatic Assay Expression Profiles
TRA 1-60
Morphology pictures

Microbiology / Virus Screening

HIV 1 Negative
HIV 2 Negative
Hepatitis B Negative
Hepatitis C Negative
Mycoplasma Negative


Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
Normal 46XX
Karyotyping method: Molecular karyotyping by SNP array

Other Genotyping (Cell Line)