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LBi008-A

PH-BL-407 Clone 128, iLB-289bms128

iPSC line

Not released.
The cell line is not released.

General#

Cell Line

hPSCreg name LBi008-A
Alternative name(s)
PH-BL-407 Clone 128, iLB-289bms128
Cell line type Human induced pluripotent stem cell (hiPSC)
Similar lines No similar lines found.
Notes CD33 protective Alzheimer disease TREM2-/- CD33-/- (TREM2WT/WT, CD33m/m)

Provider

Depositor Life & Brain GmbH (LB)
Distributors
EBiSC
Derivation country Germany

External Databases

hPSCreg LBi008-A
BioSamples SAMEA111511681
Cellosaurus CVCL_C6QW
Wikidata Q117704608

General Information

This EBiSC line can be used for:
Yes
Research use: allowed
Clinical use: no
Commercial use: no

Donor Information#

General Donor Information

Sex female
Age of donor (at collection) 75-79
Ethnicity White European

Phenotype and Disease related information (Donor)

Diseases No disease was diagnosed.
TREM2WT/WT CD33EXON2 splice variant/ EXON2 splice variant
The donor is not a carrier of a disease-associated mutation and not affected.
Stage
Cognitively normal on the day of sample donation
Synonyms
  • Late-onset form of familial Alzheimer disease
  • Alzheimer disease
Genetic variants
CD33 (target)
19q13.41
NC_000019.10:g.51224706C>A
Homozygous
CD33 (target)
19q13.41
NC_000019.10:g.51225221C>T
Homozygous
Disease associated phenotypes no phenotypes

Karyotyping (Donor)

Has the donor karyotype been analysed?
Unknown

Donor Relations

Other cell lines of this donor

External Databases (Donor)

BioSamples SAMEA111511729

hIPSC Derivation#

General

Source cell type
Committed, erythroid stem cells derived from myeloid stem cells. The progenitor cells develop in two phases: erythroid burst-forming units (bfu-e) followed by erythroid colony-forming units (cfu-e). Bfu-e differentiate into cfu-e on stimulation by erythropoietin, and then further differentiate into erythroblasts when stimulated by other factors.
Source cell origin
A liquid tissue; its major function is to transport oxygen throughout the body. It also supplies the tissues with nutrients, removes waste products, and contains various components of the immune system defending the body against infection. Several hormones also travel in the blood.
Synonyms
  • Reticuloendothelial System, Blood
  • Peripheral Blood
  • peripheral blood
  • blood
  • Blood
  • Whole Blood
  • BLOOD
  • portion of blood
  • vertebrate blood
show more synonyms
Age of donor (at collection) 75-79
Collected in 2018

Reprogramming method

Vector type Non-integrating
Vector Sendai virus
Is reprogramming vector detectable?
No
Methods used
RT-PCR
Files and images showing reprogramming vector expressed or silenced

Vector free reprogramming

Other

Derived under xeno-free conditions
No
Derived under GMP?
No
Available as clinical grade?
No

Culture Conditions#

The following are the depositor culture conditions, they do not refer to any specific batch.
Surface coating Vitronectin
Feeder cells
No
Passage method Enzymatically
Accutase
O2 Concentration 20 %
CO2 Concentration 6 %
Medium Other medium:
Base medium: StemMACS™ iPS-Brew XF, Basal Medium
Main protein source: StemMACS™ iPS Brew XF, 50x Supplement
Serum concentration: %
Has Rock inhibitor (Y27632) been used at passage previously with this cell line?
Yes
Has Rock inhibitor (Y27632) been used at cryo previously with this cell line?
No
Has Rock inhibitor (Y27632) been used at thaw previously with this cell line?
Yes

Characterisation#

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR Flow Cytometry Enzymatic Assay Expression Profiles
Morphology pictures

Microbiology / Virus Screening

HIV 1 Negative
HIV 2 Negative
Hepatitis B Negative
Hepatitis C Negative
Mycoplasma Negative

Genotyping#

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
Yes
Normal 46XY
Karyotyping method: Molecular karyotyping by SNP array
http://

Other Genotyping (Cell Line)