ISCRMi001-A
3-0062, DSP Leu462fs
iPSC line
The cell line is not available.
General#
Cell Line |
|
hPSCreg Name | ISCRMi001-A |
Alternative name(s) |
3-0062, DSP Leu462fs
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Cell line type | Human induced pluripotent stem cell (hiPSC) |
Similar lines |
UKKi035-B (NP0139-B, NP0139-6C) Donor's gene variants: MYBPC3, MYBPC3 Donor diseases: Hypertrophic cardiomyopathy SCVIi073-A (SCVIi574C2) Donor's gene variants: titin, BAG cochaperone 3, BAG cochaperone 3 Donor diseases: Dilated Cardiomyopathy SCVIi074-A (SCVIi599C1) Donor's gene variants: BAG cochaperone 3 Donor diseases: Hypertrophic Cardiomyopathy FAMRCi006-A (LMNA T3) Donor's gene variants: LMNA Donor diseases: Emery-Dreifuss Muscular Dystrophy Dilated Cardiomyopathy FAMRCi006-B (LMNA T4) Donor's gene variants: LMNA Donor diseases: Emery-Dreifuss Muscular Dystrophy Dilated Cardiomyopathy |
Provider |
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Depositor | University of Washington Institute for Stem Cell and Regenerative Medicine (ISCRM) |
Owner | University of Washington Institute for Stem Cell and Regenerative Medicine (ISCRM) |
Distributors |
EBiSC
European Collection of Authenticated Cell Cultures (ECACC)
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Derivation country | United States |
External Databases |
|
hPSCreg | ISCRMi001-A |
BioSamples | SAMEA111405406 |
CLO | CLO_0104082 |
Cellosaurus | CVCL_C6Q1 |
Wikidata | Q117704554 |
General Information |
|
Publications | View all related publications on hPSCreg (1) |
* Is the cell line readily obtainable for third parties? |
Yes Research use: allowed
Clinical use: allowed
Commercial use: not allowed
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Donor Information#
General Donor Information |
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Sex | female |
Ethnicity | White |
Phenotype and Disease related information (Donor) |
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Diseases | A disease was diagnosed.
|
Disease associated phenotypes |
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Family history | Unknown |
Is the medical history available upon request? | No |
Is clinical information available? | No |
Karyotyping (Donor) |
|
Has the donor karyotype been analysed? |
Yes
Karyotyping method:
G-Banding
|
Other Genotyping (Donor) |
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Is there genome-wide genotyping or functional data available? |
No
|
External Databases (Donor) |
|
BioSamples | SAMEA111405407 |
hIPSC Derivation#
General |
|
Source cell type |
Peripheral Blood Mononuclear CellA peripheral blood cell with a single nucleus. This category includes lymphocytes and monocytes.
Synonyms
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Passage number reprogrammed | 1 |
Reprogramming method |
|
Vector type | Integrating |
Vector | Virus (Sendaivirus) |
Is the used vector excisable? |
No |
Absence of reprogramming vector(s)? |
Yes |
Reprogramming vectors silenced? |
Yes |
Methods used |
PCR
|
Vector free reprogramming |
|
Type of used vector free reprogramming factor(s) |
None
|
Other |
|
Derived under xeno-free conditions |
No |
Derived under GMP? |
No |
Available as clinical grade? |
No |
Culture Conditions#
The following are the depositor culture conditions, they do not refer to any specific batch.
Surface coating | Matrigel/Geltrex |
Feeder cells |
No |
Passage method |
Enzyme-free cell dissociation
EDTA
|
O2 Concentration | 20 % |
CO2 Concentration | 5 % |
Medium |
mTeSR™ Plus
|
Has Rock inhibitor (Y27632) been used at passage previously with this cell line? | Yes |
Has Rock inhibitor (Y27632) been used at cryo previously with this cell line? | No |
Has Rock inhibitor (Y27632) been used at thaw previously with this cell line? | Yes |
Characterisation#
Analysis of Undifferentiated Cells
Marker | Expressed | Immunostaining | RT-PCR | FACS | Enzymatic Assay | Expression Profiles |
NANOG |
Yes |
|||||
POU5F1 (OCT-4) |
Yes |
Differentiation Potency
In vitro directed differentiation
Genotyping#
Karyotyping (Cell Line) |
|
Has the cell line karyotype been analysed? |
Yes
46XX
Passage number: 9
Karyotyping method:
G-Banding
|
Other Genotyping (Cell Line) |