The EBiSC team is working hard to implement improvements in how EBiSC operates.
Due to some short-term disruption, please get in touch via
Contact@EBiSC.org if
the cells you would like to access are currently listed as unavailable or you
are ordering from outside of Europe.
DHMi005-A-3
L_FLAG Clone 6
Gene-edited iPSC line
We are currently making some changes to how EBiSC operates and because
of this there is a short period of time where orders cannot be placed.
If you are interested in accessing these cells, please contact EBiSC directly. For more information about the current transition process see here.
If you are interested in accessing these cells, please contact EBiSC directly. For more information about the current transition process see here.
The cell line is not released.
General#
Cell Line |
|
hPSCreg name | DHMi005-A-3 |
Alternative name(s) |
L_FLAG Clone 6
|
Cell line type | Human induced pluripotent stem cell (hiPSC) |
Similar lines | No similar lines found. |
Provider |
|
Depositor | German Heart Center Munich (DHM) |
Owner | German Heart Center Munich, Department of Cardiovascular Surgery, Institute Insure |
Distributors |
German Heart Center Munich, Department of Cardiovascular Surgery, Institute Insure
|
Derivation country | Germany |
External Databases |
|
hPSCreg | DHMi005-A-3 |
BioSamples | SAMEA14491815 |
Cellosaurus | CVCL_C0IS |
Wikidata | Q112929455 |
General Information |
|
Publications | View all related publications on hPSCreg (1) |
This EBiSC line can be used for: |
Yes
Research use: allowed
Clinical use: no
Commercial use: no
|
Subclone of | (not in EBiSC, see DHMi005-A in hPSCreg) |
Donor Information#
General Donor Information |
|
Sex | male |
Ethnicity | Caucasian |
Phenotype and Disease related information (Donor) |
|
Diseases | No disease was diagnosed.
|
Family history | No abnormalities have been described with regard to the family history. |
Is the medical history available upon request? | no, healthy proband |
Is clinical information available? | no, healthy proband |
Other Genotyping (Donor) |
|
Is there genome-wide genotyping or functional data available? |
No
|
External Databases (Donor) |
|
BioSamples | SAMEA12738677 |
hIPSC Derivation#
General |
|
More source cell information can be found in the parental cell line
DHMi005-A in hPSCreg.
|
|
Reprogramming method |
|
Vector type | Non-integrating |
Vector | Sendai virus |
Is reprogramming vector detectable? |
No |
Vector free reprogramming |
|
Other |
|
Derived under xeno-free conditions |
No |
Derived under GMP? |
No |
Available as clinical grade? |
No |
Culture Conditions#
The following are the depositor culture conditions, they do not refer to any specific batch.
Surface coating | Matrigel/Geltrex |
Feeder cells |
No |
Passage method |
Enzyme-free cell dissociation
ReLeSR
|
Medium |
mTeSR™ Plus
|
Has Rock inhibitor (Y27632) been used at passage previously with this cell line? | Yes |
Characterisation#
Analysis of Undifferentiated Cells
Score:
Marker | Present | Absent |
mCpG | ||
OCT4 |
Differentiation Potency
Microbiology / Virus Screening |
|
Mycoplasma | Negative |
Genotyping#
Karyotyping (Cell Line) |
|
Has the cell line karyotype been analysed? |
Yes
46 XY
Passage number: 46
|
Other Genotyping (Cell Line) |
Genetic Modification#
Genetic modifications not related to a disease |
|