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DHMi005-A-3

L_FLAG Clone 6

Gene-edited iPSC line

The cell line is not available.

General#

Cell Line

hPSCreg Name DHMi005-A-3
Alternative name(s)
L_FLAG Clone 6
Cell line type Human induced pluripotent stem cell (hiPSC)

Provider

Depositor German Heart Center Munich (DHM)
Owner German Heart Center Munich, Department of Cardiovascular Surgery, Institute Insure
Distributors
EBiSC
European Collection of Authenticated Cell Cultures (ECACC)
German Heart Center Munich, Department of Cardiovascular Surgery, Institute Insure
Derivation country Germany

External Databases

hPSCreg DHMi005-A-3
BioSamples SAMEA14491815
CLO CLO_0103888
Cellosaurus CVCL_C0IS
Wikidata Q112929455

General Information

* Is the cell line readily obtainable for third parties?
No
Subclone of (not in EBiSC, see DHMi005-A in hPSCreg)

Donor Information#

General Donor Information

Sex male
Ethnicity Caucasian

Phenotype and Disease related information (Donor)

Diseases No disease was diagnosed.
Family history No abnormalities have been described with regard to the family history.
Is the medical history available upon request? no, healthy proband
Is clinical information available? no, healthy proband

Other Genotyping (Donor)

Is there genome-wide genotyping or functional data available?
No

External Databases (Donor)

BioSamples SAMEA12738677

hIPSC Derivation#

General

More source cell information can be found in the parental cell line DHMi005-A in hPSCreg.

Reprogramming method

Vector type Non-integrating
Vector Sendai virus
Is reprogramming vector detectable?
No

Vector free reprogramming

Other

Derived under xeno-free conditions
No
Derived under GMP?
No
Available as clinical grade?
No

Culture Conditions#

The following are the depositor culture conditions, they do not refer to any specific batch.
Surface coating Matrigel/Geltrex
Feeder cells
No
Passage method Enzyme-free cell dissociation
ReLeSR
Medium mTeSR™ Plus
Has Rock inhibitor (Y27632) been used at passage previously with this cell line?
Yes

Characterisation#

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR FACS Enzymatic Assay Expression Profiles
OCT4
Yes
KLF4
Yes
SOX2
Yes
c-MYC
Yes
NANOG
Yes
REX1
Yes
TRA 1-81
Yes
TRA 1-60
Yes
SOX2
Yes
NANOG
Yes
TRA 1-81
Yes
Score:
Marker Present Absent
mCpG
OCT4
Differentiation Potency

Microbiology / Virus Screening

Mycoplasma Negative

Genotyping#

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
Yes
46 XY
Passage number: 46

Other Genotyping (Cell Line)

Genetic Modification#

Genetic modifications not related to a disease
TBX5 (target)
Variant
12q24
NM_000192.3:c.52908_52909insGACTACAAGGACGACGATGACAAA
NP_852259:p.S518_ins.DYKDDDDK
Heterozygous
Insertion of an Flag-Taq in the last Exon of TBX5 in front of the stop codon.