CTEFi001-A
CTEF-hiPSC10, FNUSA-hiPSC10
iPSC line
The cell line is not available.
General#
Cell Line |
|
| hPSCreg Name | CTEFi001-A |
| Alternative name(s) |
CTEF-hiPSC10, FNUSA-hiPSC10
|
| Cell line type | Human induced pluripotent stem cell (hiPSC) |
Provider |
|
| Depositor | Cell and Tissue Engineering Facility (CTEF) |
| Owner | Cell and Tissue Engineering Facility (CTEF) |
| Distributors |
EBiSC
|
| Derivation country | Czech Republic |
External Databases |
|
| hPSCreg | CTEFi001-A |
| BioSamples | SAMEA113904283 |
General Information |
|
| * Is the cell line readily obtainable for third parties? |
Yes Research use: allowed
Clinical use: allowed
Commercial use: allowed
|
Donor Information#
General Donor Information |
|
| Sex | male |
| Age of donor (at collection) | 20-24 |
Phenotype and Disease related information (Donor) |
|
| Diseases | No disease was diagnosed.
|
Karyotyping (Donor) |
|
| Has the donor karyotype been analysed? |
No
|
Other Genotyping (Donor) |
|
| Is there genome-wide genotyping or functional data available? |
No
|
External Databases (Donor) |
|
| BioSamples | SAMEA113904284 |
hIPSC Derivation#
General |
|
| Source cell type |
Peripheral BloodBlood drawn from a limb.
Synonyms
|
| Source cell origin |
Peripheral BloodBlood drawn from a limb.
Synonyms
|
| Age of donor (at collection) | 20-24 |
| Collected in | 2022 |
Reprogramming method |
|
| Vector type | Non-integrating |
| Vector | Episomal |
| Is reprogramming vector detectable? |
No |
| Methods used |
RT-PCR
|
Vector free reprogramming |
|
Other |
|
| Derived under xeno-free conditions |
Yes |
| Derived under GMP? |
Yes |
| Available as clinical grade? |
Yes |
Culture Conditions#
The following are the depositor culture conditions, they do not refer to any specific batch.
| Surface coating | Vitronectin |
| Feeder cells |
No |
| Passage method |
Enzyme-free cell dissociation
EDTA
|
| O2 Concentration | 21 % |
| CO2 Concentration | 5 % |
| Medium |
Other medium:
Base medium: NutriStem® hPSC XF Media
Main protein source: Serum concentration: % |
| Has Rock inhibitor (Y27632) been used at passage previously with this cell line? | Yes |
| Has Rock inhibitor (Y27632) been used at cryo previously with this cell line? | Yes |
| Has Rock inhibitor (Y27632) been used at thaw previously with this cell line? | Yes |
Characterisation#
Analysis of Undifferentiated Cells
| Marker | Expressed | Immunostaining | RT-PCR | FACS | Enzymatic Assay | Expression Profiles |
Differentiation Potency
In vitro spontaneous differentiation
| Marker | Expressed |
| AFP |
Yes |
| FOXA2 |
Yes |
In vitro directed differentiation
| Marker | Expressed |
| Vimentin |
Yes |
| SMA |
Yes |
In vitro spontaneous differentiation
| Marker | Expressed |
| Nestin |
Yes |
| Beta III Tubulin |
Yes |
Microbiology / Virus Screening |
|
| HIV 1 | Negative |
| HIV 2 | Negative |
| Hepatitis B | Negative |
| Hepatitis C | Negative |
| Mycoplasma | Negative |
Genotyping#
Karyotyping (Cell Line) |
|
| Has the cell line karyotype been analysed? |
Yes
Normal male karyotype
Passage number: 18
Karyotyping method:
G-Banding
|
Other Genotyping (Cell Line) |
|