The EBiSC team is working hard to implement improvements in how EBiSC operates. Due to some short-term disruption, please get in touch via Contact@EBiSC.org if the cells you would like to access are currently listed as unavailable or you are ordering from outside of Europe.

CTEFi001-A

CTEF-hiPSC10, FNUSA-hiPSC10

iPSC line

Not released.
We are currently making some changes to how EBiSC operates and because of this there is a short period of time where orders cannot be placed.

If you are interested in accessing these cells, please contact EBiSC directly. For more information about the current transition process see here.
The cell line is not released.

General#

Cell Line

hPSCreg name CTEFi001-A
Alternative name(s)
CTEF-hiPSC10, FNUSA-hiPSC10
Cell line type Human induced pluripotent stem cell (hiPSC)
Similar lines No similar lines found.

Provider

Depositor Cell and Tissue Engineering Facility (CTEF)
Owner Cell and Tissue Engineering Facility (CTEF)
Derivation country Czech Republic

External Databases

hPSCreg CTEFi001-A
BioSamples SAMEA113904283
Cellosaurus CVCL_D0DT
Wikidata Q123030981

General Information

This EBiSC line can be used for:
Yes
Research use: allowed
Clinical use: no
Commercial use: no

Donor Information#

General Donor Information

Sex male
Age of donor (at collection) 20-24

Phenotype and Disease related information (Donor)

Diseases No disease was diagnosed.

Karyotyping (Donor)

Has the donor karyotype been analysed?
No

Other Genotyping (Donor)

Is there genome-wide genotyping or functional data available?
No

External Databases (Donor)

BioSamples SAMEA113904284

hIPSC Derivation#

General

Source cell type
Blood drawn from a limb.
Synonyms
  • Peripheral Blood
  • Peripheral blood
Source cell origin
Blood drawn from a limb.
Synonyms
  • Peripheral Blood
  • Peripheral blood
Age of donor (at collection) 20-24
Collected in 2022

Reprogramming method

Vector type Non-integrating
Vector Episomal
Is reprogramming vector detectable?
No
Methods used
RT-PCR

Vector free reprogramming

Other

Derived under xeno-free conditions
Yes
Derived under GMP?
Yes
Available as clinical grade?
Yes

Culture Conditions#

The following are the depositor culture conditions, they do not refer to any specific batch.
Surface coating Vitronectin
Feeder cells
No
Passage method Enzyme-free cell dissociation
EDTA
O2 Concentration 21 %
CO2 Concentration 5 %
Medium Other medium:
Base medium: NutriStem® hPSC XF Media
Main protein source:
Serum concentration: %
Has Rock inhibitor (Y27632) been used at passage previously with this cell line?
Yes
Has Rock inhibitor (Y27632) been used at cryo previously with this cell line?
Yes
Has Rock inhibitor (Y27632) been used at thaw previously with this cell line?
Yes

Characterisation#

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR Flow Cytometry Enzymatic Assay Expression Profiles
Differentiation Potency
Endoderm
Ont Id: UBERON_0000925
In vitro spontaneous differentiation
Marker Expressed
AFP
Yes
FOXA2
Yes
Mesoderm
Ont Id: UBERON_0000926
In vitro directed differentiation
Marker Expressed
Vimentin
Yes
SMA
Yes
Ectoderm
Ont Id: UBERON_0000924
In vitro spontaneous differentiation
Marker Expressed
Nestin
Yes
Beta III Tubulin
Yes

Microbiology / Virus Screening

HIV 1 Negative
HIV 2 Negative
Hepatitis B Negative
Hepatitis C Negative
Mycoplasma Negative

Genotyping#

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
Yes
Normal male karyotype
Passage number: 18
Karyotyping method: G-Banding

Other Genotyping (Cell Line)