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CSSi013-A

iPSC line

The cell line is not available.

General#

Cell Line

hPSCreg Name CSSi013-A
Cell line type Human induced pluripotent stem cell (hiPSC)

Provider

Depositor Fondazione Casa Sollievo della Sofferenza IRCCS (CSS)
Owner Fondazione Casa Sollievo della Sofferenza IRCCS (CSS)
Distributors
EBiSC
European Collection of Authenticated Cell Cultures (ECACC)
Fondazione Casa Sollievo della Sofferenza IRCCS (CSS)
Derivation country Italy

External Databases

hPSCreg CSSi013-A
BioSamples SAMEA14080160
CLO CLO_0103797
Cellosaurus CVCL_B7SG
Wikidata Q112929419

General Information

Publications View all related publications on hPSCreg (1)
* Is the cell line readily obtainable for third parties?
Yes
Research use: allowed
Clinical use: not allowed
Commercial use: not allowed

Donor Information#

General Donor Information

Sex female
Age of donor (at collection) 30-34
Ethnicity Caucasian

Phenotype and Disease related information (Donor)

Diseases No disease was diagnosed.
Disease associated phenotypes
  • Amyotrophic Lateral Sclerosis
Family history yes

Karyotyping (Donor)

Has the donor karyotype been analysed?
Yes
Karyotyping method: G-Banding

External Databases (Donor)

BioSamples SAMEA14080161

hIPSC Derivation#

General

Source cell type
Fibroblasts
Age of donor (at collection) 30-34
Collected in 2018
Passage number reprogrammed IV

Reprogramming method

Vector type Non-integrating
Vector Episomal
Is reprogramming vector detectable?
Yes
Methods used
RT-PCR
Files and images showing reprogramming vector expressed or silenced

Vector free reprogramming

Type of used vector free reprogramming factor(s)
None

Other

Selection criteria for clones marker expression, cell morphology
Derived under xeno-free conditions
Yes
Derived under GMP?
No
Available as clinical grade?
No

Culture Conditions#

The following are the depositor culture conditions, they do not refer to any specific batch.
Surface coating Matrigel/Geltrex
Feeder cells
No
Passage method Mechanically
CO2 Concentration 5 %
Medium Other medium:
Base medium: Nutristem hPSC XF medium
Main protein source:
Serum concentration: %
Has Rock inhibitor (Y27632) been used at passage previously with this cell line?
No
Has Rock inhibitor (Y27632) been used at cryo previously with this cell line?
No
Has Rock inhibitor (Y27632) been used at thaw previously with this cell line?
No

Characterisation#

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR FACS Enzymatic Assay Expression Profiles
POU5F1 (OCT-4)
Yes
Morphology pictures
CLONE.tif
iPSC morphology
Differentiation Potency
Endoderm
Ont Id: UBERON_0000925
In vivo teratoma
In vitro spontaneous differentiation
Marker Expressed
EOMES
Yes
SLC1A3
Yes
GATA4
Yes
Morphology
ENDODERMAL LAYER.tif
endodermal layer
Mesoderm
Ont Id: UBERON_0000926
In vivo teratoma
In vitro spontaneous differentiation
Marker Expressed
FOXA2
Yes
SOX17
Yes
TBXT
Yes
Morphology
MESODERMAL LAYER.tif
mesodermal layer
Ectoderm
Ont Id: UBERON_0000924
In vivo teratoma
In vitro spontaneous differentiation
Marker Expressed
NESTIN
Yes
SOX1
Yes
PAX6
Yes

Genotyping#

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
Yes
IPSC
Passage number: VII
Karyotyping method: G-Banding

Other Genotyping (Cell Line)