CSSi013-A
iPSC line
The cell line is not available.
General#
Cell Line |
|
| hPSCreg Name | CSSi013-A |
| Cell line type | Human induced pluripotent stem cell (hiPSC) |
Provider |
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| Depositor | Fondazione Casa Sollievo della Sofferenza IRCCS (CSS) |
| Owner | Fondazione Casa Sollievo della Sofferenza IRCCS (CSS) |
| Distributors |
European Collection of Authenticated Cell Cultures (ECACC)
Fondazione Casa Sollievo della Sofferenza IRCCS (CSS)
|
| Derivation country | Italy |
External Databases |
|
| hPSCreg | CSSi013-A |
| BioSamples | SAMEA14080160 |
| CLO | CLO_0103797 |
| Cellosaurus | CVCL_B7SG |
| Wikidata | Q112929419 |
General Information |
|
| Publications | View all related publications on hPSCreg (1) |
| * Is the cell line readily obtainable for third parties? |
Yes Research use: allowed
Clinical use: not allowed
Commercial use: not allowed
|
Donor Information#
General Donor Information |
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| Sex | female |
| Age of donor (at collection) | 30-34 |
| Ethnicity | Caucasian |
Phenotype and Disease related information (Donor) |
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| Diseases | No disease was diagnosed.
|
| Disease associated phenotypes |
|
| Family history | yes |
Karyotyping (Donor) |
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| Has the donor karyotype been analysed? |
Yes
Karyotyping method:
G-Banding
|
External Databases (Donor) |
|
| BioSamples | SAMEA14080161 |
hIPSC Derivation#
General |
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| Source cell type |
Fibroblasts |
| Age of donor (at collection) | 30-34 |
| Collected in | 2018 |
| Passage number reprogrammed | IV |
Reprogramming method |
|
| Vector type | Non-integrating |
| Vector | Episomal |
| Is reprogramming vector detectable? |
Yes |
| Methods used |
RT-PCR
|
| Files and images showing reprogramming vector expressed or silenced | |
Vector free reprogramming |
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| Type of used vector free reprogramming factor(s) |
None
|
Other |
|
| Selection criteria for clones | marker expression, cell morphology |
| Derived under xeno-free conditions |
Yes |
| Derived under GMP? |
No |
| Available as clinical grade? |
No |
Culture Conditions#
The following are the depositor culture conditions, they do not refer to any specific batch.
| Surface coating | Matrigel/Geltrex |
| Feeder cells |
No |
| Passage method | Mechanically |
| CO2 Concentration | 5 % |
| Medium |
Other medium:
Base medium: Nutristem hPSC XF medium
Main protein source: Serum concentration: % |
| Has Rock inhibitor (Y27632) been used at passage previously with this cell line? | No |
| Has Rock inhibitor (Y27632) been used at cryo previously with this cell line? | No |
| Has Rock inhibitor (Y27632) been used at thaw previously with this cell line? | No |
Characterisation#
Analysis of Undifferentiated Cells
| Marker | Expressed | Immunostaining | RT-PCR | FACS | Enzymatic Assay | Expression Profiles |
| POU5F1 (OCT-4) |
Yes |
Morphology pictures
CLONE.tif
iPSC morphology
Differentiation Potency
In vivo teratoma
In vitro spontaneous differentiation
Morphology
Genotyping#
Karyotyping (Cell Line) |
|
| Has the cell line karyotype been analysed? |
Yes
|
Other Genotyping (Cell Line) |
|