CSSi013-A
iPSC line
The cell line is not available.
General#
Cell Line |
|
hPSCreg Name | CSSi013-A |
Cell line type | Human induced pluripotent stem cell (hiPSC) |
Provider |
|
Depositor | Fondazione Casa Sollievo della Sofferenza IRCCS (CSS) |
Owner | Fondazione Casa Sollievo della Sofferenza IRCCS (CSS) |
Distributors |
European Collection of Authenticated Cell Cultures (ECACC)
Fondazione Casa Sollievo della Sofferenza IRCCS (CSS)
|
Derivation country | Italy |
External Databases |
|
hPSCreg | CSSi013-A |
BioSamples | SAMEA14080160 |
CLO | CLO_0103797 |
Cellosaurus | CVCL_B7SG |
Wikidata | Q112929419 |
General Information |
|
Publications | View all related publications on hPSCreg (1) |
* Is the cell line readily obtainable for third parties? |
Yes Research use: allowed
Clinical use: not allowed
Commercial use: not allowed
|
Donor Information#
General Donor Information |
|
Sex | female |
Age of donor (at collection) | 30-34 |
Ethnicity | Caucasian |
Phenotype and Disease related information (Donor) |
|
Diseases | No disease was diagnosed.
|
Disease associated phenotypes |
|
Family history | yes |
Karyotyping (Donor) |
|
Has the donor karyotype been analysed? |
Yes
Karyotyping method:
G-Banding
|
External Databases (Donor) |
|
BioSamples | SAMEA14080161 |
hIPSC Derivation#
General |
|
Source cell type |
Fibroblasts |
Age of donor (at collection) | 30-34 |
Collected in | 2018 |
Passage number reprogrammed | IV |
Reprogramming method |
|
Vector type | Non-integrating |
Vector | Episomal |
Is reprogramming vector detectable? |
Yes |
Methods used |
RT-PCR
|
Files and images showing reprogramming vector expressed or silenced | |
Vector free reprogramming |
|
Type of used vector free reprogramming factor(s) |
None
|
Other |
|
Selection criteria for clones | marker expression, cell morphology |
Derived under xeno-free conditions |
Yes |
Derived under GMP? |
No |
Available as clinical grade? |
No |
Culture Conditions#
The following are the depositor culture conditions, they do not refer to any specific batch.
Surface coating | Matrigel/Geltrex |
Feeder cells |
No |
Passage method | Mechanically |
CO2 Concentration | 5 % |
Medium |
Other medium:
Base medium: Nutristem hPSC XF medium
Main protein source: Serum concentration: % |
Has Rock inhibitor (Y27632) been used at passage previously with this cell line? | No |
Has Rock inhibitor (Y27632) been used at cryo previously with this cell line? | No |
Has Rock inhibitor (Y27632) been used at thaw previously with this cell line? | No |
Characterisation#
Analysis of Undifferentiated Cells
Marker | Expressed | Immunostaining | RT-PCR | FACS | Enzymatic Assay | Expression Profiles |
POU5F1 (OCT-4) |
Yes |
Morphology pictures
CLONE.tif
iPSC morphology
Differentiation Potency
In vivo teratoma
In vitro spontaneous differentiation
Morphology
Genotyping#
Karyotyping (Cell Line) |
|
Has the cell line karyotype been analysed? |
Yes
|
Other Genotyping (Cell Line) |