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CIGLi001-A-1

SYNTHEGO 802-30F MUT, iPSC TBX4 MUT

Gene-edited iPSC line

Not-for-profit fee: £1700 per vial
Not released.
The cell line is not released.

General#

Cell Line

hPSCreg name CIGLi001-A-1
Alternative name(s)
SYNTHEGO 802-30F MUT, iPSC TBX4 MUT
Cell line type Human induced pluripotent stem cell (hiPSC)
Similar lines No similar lines found.
Notes This cell line is a subclone of CIGLi001-A and has a knockin mutation in the TBX4 gene in exon 3. The mutation: Ile119Asn fsX6 (ATT>AATT) heterozygous

Provider

Depositor Center for Infection and Genomics of the Lung (CIGL)
Derivation country United States

External Databases

hPSCreg CIGLi001-A-1
BioSamples SAMEA114705109

General Information

This EBiSC line can be used for:
Yes
Research use: allowed
Clinical use: no
Commercial use: no
Subclone of (not in EBiSC, see CIGLi001-A in hPSCreg)

Donor Information#

General Donor Information

Sex female
Ethnicity Hispanic

Phenotype and Disease related information (Donor)

Diseases No disease was diagnosed.
Family history no

Other Genotyping (Donor)

Is there genome-wide genotyping or functional data available?
Yes
Whole genome sequencing
Passes test for quality control as mentioned by supplier
vcf File:
802 30F datasheet.pdf
The company, SYNTHEGO, that is supplying the cell line to us, mentions that quality control assessment is done and sequence validation data can be provided upon request

Donor Relations

Other cell lines of this donor

External Databases (Donor)

BioSamples SAMEA114652490

hIPSC Derivation#

General

More source cell information can be found in the parental cell line CIGLi001-A in hPSCreg.
Passage number reprogrammed 34

Reprogramming method

Vector type None

Vector free reprogramming

Type of used vector free reprogramming factor(s)
None

Other

Selection criteria for clones SYNTHEGO edited the cell line using CRISPR with a single guide RNA. They used single-cell cloning using cell printer to isolate the clones.
Derived under xeno-free conditions
Yes
Derived under GMP?
Yes
Available as clinical grade?
Unknown

Culture Conditions#

The following are the depositor culture conditions, they do not refer to any specific batch.
Surface coating iMatrix added in medium
Feeder cells
No
Passage method Enzymatically
Accutase
O2 Concentration 21 %
CO2 Concentration 5 %
Medium Other medium:
Base medium: StemMACS™ iPS-Brew XF, human
Main protein source:
Serum concentration: 0 %
Supplements
StemMACS iPS-Brew XF, 50× Supplement 10 ml %
Has Rock inhibitor (Y27632) been used at passage previously with this cell line?
Yes
Has Rock inhibitor (Y27632) been used at thaw previously with this cell line?
Yes

Characterisation#

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR Flow Cytometry Enzymatic Assay Expression Profiles
NANOG
Yes
SSEA-4
Yes
TRA 1-60
Yes
Marker Present Absent
mCpG
OCT4 X
Report
Pluripotency Score Novelty Score
37.18372 1.282409
Morphology pictures
802 30F datasheet.pdf
Figure 4 - The cells growing in colonies

Microbiology / Virus Screening

HIV 1 Negative
HIV 2 Negative
Hepatitis B Negative
Hepatitis C Negative
Mycoplasma Negative

Genotyping#

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
Yes
46, XX
Passage number: P14
Karyotyping method: G-Banding

Other Genotyping (Cell Line)

Is there genome-wide genotyping or functional data available?
Yes
Sanger sequencing
Mutation is confirmed according to the analysis done by SYNTHEGO. Alighment results can be provided upon request.
vcf File:
802 30F datasheet.pdf
The company, SYNTHEGO, that is supplying the cell line to us, mentions that quality control assessment is done and sequence validation data can be provided upon request
SNP typing array
KaryoStat+ indicates 802-30F cells are genomically stable
vcf File:
802 30F datasheet.pdf
Figure 1. KaryoStat+ indicates 802-30F cells are genomically stable.* KaryoStat+ results for wild type 802-30F cells (passage 19) indicate that genomic integrity is maintained. The whole-genome view displays all somatic and sex chromosomes in one frame with a high-level copy number. The smooth signal plot (right y-axis) is the smoothing of the log2 ratios which depict the signal intensities of probes on the microarray. A value of 2 represents a normal copy number state (CN = 2). A value of 3 represents chromosomal gain (CN = 3). A value of 1 represents a chromosomal loss (CN = 1). The pink, green and yellow colors indicate the raw signal for each individual chromosome probe, while the blue signal represents the normalized probe signal which is used to identify copy number and aberrations (if any). * descriptions adapted from Thermo Fisher Scientific.

Genetic Modification#

Disease/phenotype related modifications
Genetic modifications
TBX4 (target)
Gene knock-in
Chromosome 17 and cytoband: q23.2
This cell line is a subclone of CIGLi001-A and has a knockin mutation in the TBX4 gene in exon 3. The mutation: Ile119Asn fsX6 (ATT>AATT) heterozygous
CRISPR-associated (CRISPR/Cas) System