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CIGLi001-A

RPChiPS8023G1, iPSC TBX4 WT, StemRNA™ Human iPSC 802-3G, SYNTHEGO 802-30F

iPSC line

Not released.
We are currently making some changes to how EBiSC operates and because of this there is a short period of time where orders cannot be placed.

If you are interested in accessing these cells, please contact EBiSC directly. For more information about the current transition process see here.
The cell line is not released.

General#

Cell Line

hPSCreg name CIGLi001-A
Alternative name(s)
RPChiPS8023G1, iPSC TBX4 WT, StemRNA™ Human iPSC 802-3G, SYNTHEGO 802-30F
Cell line type Human induced pluripotent stem cell (hiPSC)
Similar lines No similar lines found.
Notes Synthego supplies the following iPS cell line from an ethically sourced female donor that has been reprogrammed by REPROCELL® from blood-derived endothelial progenitor cells (EPCs) using mRNA (StemRNA™ 3rd-Gen technology)

Provider

Depositor Center for Infection and Genomics of the Lung (CIGL)
Distributors
ReproCELL Inc. (RPC)
Derivation country United States

External Databases

hPSCreg CIGLi001-A
BioSamples SAMEA114652059

General Information

This EBiSC line can be used for:
Yes
Research use: allowed
Clinical use: no
Commercial use: no
Subclones

Donor Information#

General Donor Information

Sex female
Age of donor (at collection) 30-34
Ethnicity Hispanic

Phenotype and Disease related information (Donor)

Diseases No disease was diagnosed.
Disease associated phenotypes no phenotypes
Family history no

Karyotyping (Donor)

Has the donor karyotype been analysed?
Unknown

Other Genotyping (Donor)

Is there genome-wide genotyping or functional data available?
Yes
Whole genome sequencing
Passes test for quality control as mentioned by supplier
vcf File:
802 30F datasheet.pdf
The company, SYNTHEGO, that is supplying the cell line to us, mentions that quality control assessment is done and sequence validation data can be provided upon request

Donor Relations

Other cell lines of this donor

External Databases (Donor)

BioSamples SAMEA114652490

hIPSC Derivation#

General

Source cell line name Peripheral blood-derived endothelial progenitor cells (EPCs)
Source cell type (free text) Peripheral blood-derived endothelial progenitor cells (EPCs)
Age of donor (at collection) 30-34
Collected in 2015
Source cell line vendor REPROCELL
Passage number reprogrammed 14

Reprogramming method

Vector type None

Vector free reprogramming

Type of used vector free reprogramming factor(s)
mRNA, miRNA
mRNA

Other

Selection criteria for clones Day 6, 8, 13 primary EPC-RNA-iPSC colonies were identified using Stemgent StainAlive TRA-1-60 antibody and isolated from the primary culture by Day 12-14
Derived under xeno-free conditions
Yes
Derived under GMP?
Yes
Available as clinical grade?
Unknown

Culture Conditions#

The following are the depositor culture conditions, they do not refer to any specific batch.
Surface coating iMatrix added in medium
Feeder cells
No
Passage method Enzymatically
Accutase
O2 Concentration 21 %
CO2 Concentration 5 %
Medium Other medium:
Base medium: StemMACS™ iPS-Brew XF, human
Main protein source:
Serum concentration: 0 %
Supplements
StemMACS iPS-Brew XF, 50× Supplement 10 ml %
Has Rock inhibitor (Y27632) been used at passage previously with this cell line?
Yes
Has Rock inhibitor (Y27632) been used at cryo previously with this cell line?
Unknown
Has Rock inhibitor (Y27632) been used at thaw previously with this cell line?
Yes

Characterisation#

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR Flow Cytometry Enzymatic Assay Expression Profiles
NANOG
Yes
SSEA-4
Yes
TRA 1-60
Yes
Marker Present Absent
mCpG
OCT4 X
Report
802 30F datasheet.pdf
Figure 4 - Oct4 expression
Pluripotency Score Novelty Score
37.18372 1.282409
Morphology pictures
802 30F datasheet.pdf
Figure 4 - The cells growing in colonies
Self-renewal
Unknown
Endoderm
Positive
Mesoderm
Positive
Ectoderm score
Positive
Data analysis report
Tests for iPSC-REPROCELL StemRNA-Human-iPSCs_US.pdf
The cells differentiate into all 3 germ layers
Differentiation Potency
Gut epithelium
Morphology
Tests for iPSC-REPROCELL StemRNA-Human-iPSCs_US.pdf
Page 2 - StemRNA human iPSCs differentiate in vivo into all three germ layers
Gut connective tissue
Morphology
Tests for iPSC-REPROCELL StemRNA-Human-iPSCs_US.pdf
Page 2 - StemRNA human iPSCs differentiate in vivo into all three germ layers
Epidermis and hair follicle
Morphology
Tests for iPSC-REPROCELL StemRNA-Human-iPSCs_US.pdf
Page 2 - StemRNA human iPSCs differentiate in vivo into all three germ layers

Microbiology / Virus Screening

HIV 1 Negative
HIV 2 Negative
Hepatitis B Negative
Hepatitis C Negative
Mycoplasma Negative

Genotyping#

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
Yes
46, XX
Passage number: P14
Karyotyping method: G-Banding

Other Genotyping (Cell Line)

Is there genome-wide genotyping or functional data available?
Yes
Whole genome sequencing
Passes test for quality control as mentioned by supplier
vcf File:
802 30F datasheet.pdf
The company, SYNTHEGO, that is supplying the cell line to us, mentions that quality control assessment is done and sequence validation data can be provided upon request
Tests for iPSC-REPROCELL StemRNA-Human-iPSCs_US.pdf
The company, REPROCELL; that supplies the cell line confirmed in this document that they did whole genome sequencing and that it passes the test
SNP typing array
KaryoStat+ indicates 802-30F cells are genomically stable
vcf File:
802 30F datasheet.pdf
Figure 1. KaryoStat+ indicates 802-30F cells are genomically stable.* KaryoStat+ results for wild type 802-30F cells (passage 19) indicate that genomic integrity is maintained. The whole-genome view displays all somatic and sex chromosomes in one frame with a high-level copy number. The smooth signal plot (right y-axis) is the smoothing of the log2 ratios which depict the signal intensities of probes on the microarray. A value of 2 represents a normal copy number state (CN = 2). A value of 3 represents chromosomal gain (CN = 3). A value of 1 represents a chromosomal loss (CN = 1). The pink, green and yellow colors indicate the raw signal for each individual chromosome probe, while the blue signal represents the normalized probe signal which is used to identify copy number and aberrations (if any). * descriptions adapted from Thermo Fisher Scientific.