BIONi010-C-66
BIONi010-C-N7
Gene-edited iPSC line
The cell line is not available.
General#
Cell Line |
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hPSCreg Name | BIONi010-C-66 |
Alternative name(s) |
BIONi010-C-N7
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Cell line type | Human induced pluripotent stem cell (hiPSC) |
Provider |
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Depositor | Fraunhofer Institute for Biomedical Engineering IBMT (IBMT) |
Owner | Fraunhofer Institute for Biomedical Engineering IBMT |
Distributors |
EBiSC
European Collection of Authenticated Cell Cultures (ECACC)
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Derivation country | Germany |
External Databases |
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hPSCreg | BIONi010-C-66 |
BioSamples | SAMEA111454172 |
CLO | CLO_0104114 |
General Information |
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* Is the cell line readily obtainable for third parties? |
Yes Research use: allowed
Clinical use: not allowed
Commercial use: not allowed
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Subclone of |
Donor Information#
General Donor Information |
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Sex | male |
Ethnicity | Black or African-American |
Phenotype and Disease related information (Donor) |
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Diseases | No disease was diagnosed.
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Karyotyping (Donor) |
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Has the donor karyotype been analysed? |
No
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Other Genotyping (Donor) |
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Is there genome-wide genotyping or functional data available? |
No
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Donor Relations |
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Other cell lines of this donor | |
External Databases (Donor) |
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BioSamples | SAMEA3105780 |
hIPSC Derivation#
General |
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The source cell information can be found in the parental cell line
BIONi010-C.
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Passage number reprogrammed | 48 |
Reprogramming method |
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Vector type | Non-integrating |
Vector | Episomal |
Is reprogramming vector detectable? |
No |
Methods used |
RT-PCR
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Vector free reprogramming |
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Other |
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Derived under xeno-free conditions |
No |
Derived under GMP? |
No |
Available as clinical grade? |
No |
Culture Conditions#
The following are the depositor culture conditions, they do not refer to any specific batch.
Surface coating | Matrigel/Geltrex |
Feeder cells |
No |
Passage method |
Enzyme-free cell dissociation
EDTA
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O2 Concentration | 5 % |
CO2 Concentration | 5 % |
Medium |
mTeSR™ 1
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Has Rock inhibitor (Y27632) been used at passage previously with this cell line? | Unknown |
Has Rock inhibitor (Y27632) been used at cryo previously with this cell line? | Unknown |
Has Rock inhibitor (Y27632) been used at thaw previously with this cell line? | Yes |
Characterisation#
Microbiology / Virus Screening |
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Mycoplasma | Negative |
Genotyping#
Karyotyping (Cell Line) |
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Has the cell line karyotype been analysed? |
Yes
Karyotype analysis revealed no chromosomal abnormalities
Passage number: 48
Karyotyping method:
KaryoStat
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Other Genotyping (Cell Line) |
Genetic Modification#
Disease/phenotype related modifications |
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