BIHi250-A

iPSC line

This (hPSCreg) cell line is not part of EBiSC.

General#

Cell Line

hPSCreg Name BIHi250-A
Cell line type Human induced pluripotent stem cell (hiPSC)

Provider

Depositor Berlin Institute of Health (BIH)
Owner Berlin Institute of Health (BIH)
Distributors
Berlin Institute of Health (BIH)
Derivation country Germany

External Databases

hPSCreg BIHi250-A
BioSamples SAMEA6654881
Cellosaurus CVCL_YN69
CLO CLO_0100555
Wikidata Q93426659

General Information

* Is the cell line readily obtainable for third parties?
Yes
Research use: allowed
Clinical use: not allowed
Commercial use: not allowed
Subclones

Donor Information#

General Donor Information

Sex female
Age of donor (at collection) 45-49

Phenotype and Disease related information (Donor)

Diseases No disease was diagnosed.

Donor Relations

Other cell lines of this donor

External Databases (Donor)

BioSamples SAMEA6654882

hIPSC Derivation#

General

Source cell line name CC-2511
Derived from same source line (potentially other lot and donor, see below):
Source cell type
fibroblast of dermis
Source cell origin
zone of skin
Any portion of the organ that covers that body and consists of a layer of epidermis and a layer of dermis.
Source cell type (free text) NHDF-Ad-Der Fibroblasts
Source cell line lot number
0000477954
Age of donor (at collection) 45-49
Collected in 2015
Source cell line vendor LONZA

Reprogramming method

Vector type Non-integrating
Vector ReproRNA™-OKSGM vector
Genes
Is reprogramming vector detectable?
No
Methods used
RT-PCR

Vector free reprogramming

Other

Selection criteria for clones Morphology
Derived under xeno-free conditions
No
Derived under GMP?
No
Available as clinical grade?
No

Culture Conditions#

The following are the depositor culture conditions, they do not refer to any specific batch.
Surface coating Matrigel/Geltrex
Feeder cells
No
Passage method Enzyme-free cell dissociation
EDTA
O2 Concentration 5 %
CO2 Concentration 5 %
Medium Essential 8™
Has Rock inhibitor (Y27632) been used at passage previously with this cell line?
Yes
Has Rock inhibitor (Y27632) been used at cryo previously with this cell line?
Unknown
Has Rock inhibitor (Y27632) been used at thaw previously with this cell line?
Yes

Characterisation#

Analysis of Undifferentiated Cells
Marker Expressed Immunostaining RT-PCR FACS Enzymatic Assay Expression Profiles
POU5F1 (OCT-4)
Yes
TRA 1-60
Yes
NANOG
Yes
SSEA-4
Yes
SSEA-1
No
Differentiation Potency
Endoderm
Ont Id: UBERON_0000925
In vitro directed differentiation
Mesoderm
Ont Id: UBERON_0000926
In vitro directed differentiation
Ectoderm
Ont Id: UBERON_0000924
In vitro directed differentiation

Genotyping#

Karyotyping (Cell Line)

Has the cell line karyotype been analysed?
Yes
46;XX

Other Genotyping (Cell Line)